BMRB Entry 5955
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5955
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Title: Backbone and sidechain assignment of human VASP EVH2 domain PubMed: 12220179
Deposition date: 2003-09-18 Original release date: 2003-12-19
Authors: Zimmermann, Juergen; Oschkinat, Hartmut; Ball, Linda
Citation: Zimmermann, Juergen; Labudde, Dirk; Jarchau, Thomas; Walter, Ulrich; Oschkinat, Hartmut; Ball, Linda. "Relaxation, equilibrium oligomerization and molecular symmetry of the VASP (336-380) EVH2 tetramer" Biochemistry 41, 11143-11151 (2002).
Assembly members:
Human VASP EVH2 domain, polymer, 47 residues, 5288 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Human VASP EVH2 domain: GSPSSSDYSDLQRVKQELLE
EVKKELQKVKEEIIEAFVQE
LRKRGSP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 148 |
| 15N chemical shifts | 49 |
| 1H chemical shifts | 270 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VASP EVH2 domain, chain 1 | 1 |
| 2 | VASP EVH2 domain, chain 2 | 1 |
| 3 | VASP EVH2 domain, chain 3 | 1 |
| 4 | VASP EVH2 domain, chain 4 | 1 |
Entities:
Entity 1, VASP EVH2 domain, chain 1 47 residues - 5288 Da.
| 1 | GLY | SER | PRO | SER | SER | SER | ASP | TYR | SER | ASP | ||||
| 2 | LEU | GLN | ARG | VAL | LYS | GLN | GLU | LEU | LEU | GLU | ||||
| 3 | GLU | VAL | LYS | LYS | GLU | LEU | GLN | LYS | VAL | LYS | ||||
| 4 | GLU | GLU | ILE | ILE | GLU | ALA | PHE | VAL | GLN | GLU | ||||
| 5 | LEU | ARG | LYS | ARG | GLY | SER | PRO |
Samples:
Sample_1: Human VASP EVH2 domain, [U-15N], 4.5 mM
Sample_2: Human VASP EVH2 domain, [U-15N; U-13C], 4.0 mM
Cond_1: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-NOESY-HSQC | not available | not available | not available |
| 13C-NOESY-HSQC | not available | not available | not available |
| 13C-HMQC-NOESY | not available | not available | not available |
| CBCACONNH | not available | not available | not available |
| CBCANNH | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
| 15N-TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
Software:
xwinnmr v2.6 - data collection, processing
ANSIG v3.3 - assignment, spectra handling
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG37336 BAJ21098 |
| EMBL | CAA67147 CAA86523 |
| GB | AAH26019 AAH38224 AIC59345 EAW57362 EAW57363 |
| REF | NP_003361 XP_003817563 XP_005259256 XP_005259257 XP_008968979 |
| SP | P50552 |
| AlphaFold | P50552 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts