BMRB Entry 5968
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5968
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: NMR structure of the human dematin headpiece domain PubMed: 14660664
Deposition date: 2003-10-13 Original release date: 2004-02-12
Authors: Frank, B.; Vardar, D.; Chishti, A.; McKnight, C.
Citation: Frank, B.; Vardar, D.; Chishti, A.; McKnight, C.. "The NMR Structure Dematin Headpiece Reveals a Dynamic Loop that is Conformationally Altered Upon Phosphorylation at a Distal Site " J. Biol. Chem. ., .-..
Assembly members:
dematin, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
dematin: PGLQIYPYEMLVVTNKGRTK
LPPGVDRMRLERHLSAEDFS
RVFAMSPEEFGKLALWKRNE
LKKKASLF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 487 |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dematin | 1 |
Entities:
Entity 1, dematin 68 residues - Formula weight is not available
| 1 | PRO | GLY | LEU | GLN | ILE | TYR | PRO | TYR | GLU | MET | ||||
| 2 | LEU | VAL | VAL | THR | ASN | LYS | GLY | ARG | THR | LYS | ||||
| 3 | LEU | PRO | PRO | GLY | VAL | ASP | ARG | MET | ARG | LEU | ||||
| 4 | GLU | ARG | HIS | LEU | SER | ALA | GLU | ASP | PHE | SER | ||||
| 5 | ARG | VAL | PHE | ALA | MET | SER | PRO | GLU | GLU | PHE | ||||
| 6 | GLY | LYS | LEU | ALA | LEU | TRP | LYS | ARG | ASN | GLU | ||||
| 7 | LEU | LYS | LYS | LYS | ALA | SER | LEU | PHE |
Samples:
sample_1: dematin, [U-13C; U-15N], 1 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%
sample_2: dematin, [U-15N], 4 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%
sample_3: dematin, [U-10% 13C; U-15N], 0.7 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%
sample_cond_1: pH: 6.0; temperature: 293 K; ionic strength: 10 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACO | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 15N-separated TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
CNS v1.0 - structure solution
NMRPipe vsgi6x - processing
XWINNMR v3.1 - collection
NMRView v4.1.2 - data analysis
MOLMOL v2k.2 - refinement
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 6718 |
| PDB | |
| DBJ | BAD92955 BAE28799 BAE34638 BAE37092 BAE38123 |
| EMBL | CAH92510 CAH93265 CAH93400 |
| GB | AAA58438 AAC50223 AAD34233 AAD38412 AAH06318 |
| REF | NP_001029603 NP_001107607 NP_001107608 NP_001107609 NP_001107610 |
| SP | Q08495 Q5R4B6 Q9WV69 |
| TPG | DAA26732 |
| AlphaFold | Q08495 Q5R4B6 Q9WV69 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts