BMRB Entry 6002
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6002
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Structure of the C-terminal domain of p63 PubMed: 16679535
Deposition date: 2003-11-11 Original release date: 2007-03-22
Authors: Cadot, Bruno; Candi, Eleonora; Cicero, Daniel; Desideri, Alesssandro; Mele, Sonia; Melino, Gerry; Paci, Maurizio
Citation: Cicero, Daniel; Falconi, M.; Candi, Eleonora; Mele, Sonia; Cadot, Bruno; Di Venere, A.; Rufini, S.; Melino, Gerry; Desideri, Alessandro. "NMR structure of the p63 SAM domain and dynamical properties of G534V and T537P pathological mutants, identified in the AEC syndrome" Cell Biochem. Biophys. 44, 475-489 (2006).
Assembly members:
polymer, polymer, 75 residues, 8379 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEXsx2
Entity Sequences (FASTA):
polymer: PPPYPTDCSIVSFLARLGCS
SCLDYFTTQGLTTIYQIEHY
SMDDLASLKIPEQFRHAIWK
GILDHRQLHEFAAAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 337 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 507 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p63 monomer | 1 |
Entities:
Entity 1, p63 monomer 75 residues - 8379 Da.
| 1 | PRO | PRO | PRO | TYR | PRO | THR | ASP | CYS | SER | ILE | ||||
| 2 | VAL | SER | PHE | LEU | ALA | ARG | LEU | GLY | CYS | SER | ||||
| 3 | SER | CYS | LEU | ASP | TYR | PHE | THR | THR | GLN | GLY | ||||
| 4 | LEU | THR | THR | ILE | TYR | GLN | ILE | GLU | HIS | TYR | ||||
| 5 | SER | MET | ASP | ASP | LEU | ALA | SER | LEU | LYS | ILE | ||||
| 6 | PRO | GLU | GLN | PHE | ARG | HIS | ALA | ILE | TRP | LYS | ||||
| 7 | GLY | ILE | LEU | ASP | HIS | ARG | GLN | LEU | HIS | GLU | ||||
| 8 | PHE | ALA | ALA | ALA | SER |
Samples:
sample_1: polymer, [U-15N], 0.75 mM; sodium posphate 25 mM; sodium chloride 150 mM; AEBSF 1 mM; DTT 3 mM
sample_2: polymer, [U-13C; U-15N], 0.25 mM; sodium posphate 25 mM; sodium chloride 150 mM; AEBSF 1 mM; DTT 3 mM
Exp-cond_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D CBCA(co)NH | not available | not available | not available |
| 3D CBCANH | not available | not available | not available |
| 3D HBHA(co)NH | not available | not available | not available |
| 3D HACACO | not available | not available | not available |
| 2D HACA | not available | not available | not available |
| 3D hCCH TOCSY | not available | not available | not available |
| 3D HcCH TOCSY | not available | not available | not available |
| 3D HcCH COSY | not available | not available | not available |
| 3D hCCH COSY | not available | not available | not available |
| 3D C13,N15 edited NOESY | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
Software:
NMRView v5 - peak-picking analysis of processed data
InsightII - graphic processing
X-PLOR v3.8 - Structure calculation
Procheck NMR - Structure validation
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 400 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts