BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6033

Title: NMR characterisation shows that a b-type variant of Hydrogenobacter thermophilus cytochrome c552 retains its native fold   PubMed: 14726539

Deposition date: 2003-12-05 Original release date: 2004-02-12

Authors: Wain, Rachel; Redfield, Christina; Smith, Lorna

Citation: Wain, Rachel; Redfield, Christina; Smith, Lorna. "NMR analysis shows that a b-type variant of hydrogenobacter thermophilus cytochrome c552 retains its native structure "  J. Biol. Chem. ., .-..

Assembly members:
Cytochrome c552 from Hydrogenobacter thermophilus, polymer, 80 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Hydrogenobacter thermophilus   Taxonomy ID: 940   Superkingdom: Archaea   Kingdom: not available   Genus/species: Hydrogenobacter thermophilus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cytochrome c552 from Hydrogenobacter thermophilus: NEQLAKQKGAMAAHDLKAKK VGPAYADVAKKYAGRKDAVD YLAGKIKKGGSGVWGSVPMP PQNVTDAEAKQLAQWILSIK

Data sets:
Data typeCount
1H chemical shifts283
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C10A/C13A cytochrome c5521
2PROTOPORPHYRIN IX CONTAINING FE2

Entities:

Entity 1, C10A/C13A cytochrome c552 80 residues - Formula weight is not available

1   ASNGLUGLNLEUALALYSGLNLYSGLYALA
2   METALAALAHISASPLEULYSALALYSLYS
3   VALGLYPROALATYRALAASPVALALALYS
4   LYSTYRALAGLYARGLYSASPALAVALASP
5   TYRLEUALAGLYLYSILELYSLYSGLYGLY
6   SERGLYVALTRPGLYSERVALPROMETPRO
7   PROGLNASNVALTHRASPALAGLUALALYS
8   GLNLEUALAGLNTRPILELEUSERILELYS

Entity 2, PROTOPORPHYRIN IX CONTAINING FE - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome c552 from Hydrogenobacter thermophilus, [U-15N], 1.0 mM

conditions_sample_1: pH: 7.4 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableconditions_sample_1
1H-15N TOCSYsample_1not availableconditions_sample_1
1H-15N HSQCsample_1not availableconditions_sample_1
1H-15N HMQCJsample_1not availableconditions_sample_1

Software:

No software information available

NMR spectrometers:

  • Homemade . 750 MHz

Related Database Links:

BMRB 10134 10135 17053 5086 5087 5088 5089
PDB
DBJ BAI69446
EMBL CAA40902
GB ADO45379
REF WP_012963626 YP_003432647 YP_005511672
SP P15452
AlphaFold P15452

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts