BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6050

Title: Solution structure of Sso7d mutant (K12L), NMR, 4 structures   PubMed: 17340638

Deposition date: 2003-12-16 Original release date: 2007-11-05

Authors: Recca, T.; Consonni, R.; Alberti, E.; Arosio, I.; Zetta, L.

Citation: Consonni, R.; Arosio, I.; Recca, T.; Fusi, P.; Zetta, L.. "Structural determinants responsible for the thermostability of Sso7d and its single point mutants"  Proteins 67, 766-775 (2007).

Assembly members:
SSO7D, polymer, 62 residues, Formula weight is not available

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SSO7D: ATVKFKYKGEELQVDISKIK KVWRVGKMISFTYDEGGGKT GRGAVSEKDAPKKLLQMLEK QK

Data sets:
Data typeCount
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Endoribounuclease SSO7D1

Entities:

Entity 1, Endoribounuclease SSO7D 62 residues - Formula weight is not available

1   ALATHRVALLYSPHELYSTYRLYSGLYGLU
2   GLULEUGLNVALASPILESERLYSILELYS
3   LYSVALTRPARGVALGLYLYSMETILESER
4   PHETHRTYRASPGLUGLYGLYGLYLYSTHR
5   GLYARGGLYALAVALSERGLULYSASPALA
6   PROLYSLYSLEULEUGLNMETLEUGLULYS
7   GLNLYS

Samples:

sample_1: SSO7D 2 mM; D2O 10%; H2O 90%

sample_2: SSO7D 2 mM; D2O 100%; H2O 90%

sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

FELIX v98.0 - data analysis

DISCOVER v3.0 - refinement, structure solution

xwinnmr v2.6 - collection

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB