BMRB Entry 6069
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6069
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Title: Chemical Shift Assignments for Precarnobacteriocin B2 PubMed: 15096213
Deposition date: 2004-01-08 Original release date: 2004-06-30
Authors: Sprules, Tara; Kawulka, Karen; Gibbs, Alan; Wishart, David; Vederas, John
Citation: Sprules, Tara; Kawulka, Karen; Gibbs, Alan; Wishart, David; Vederas, John. "NMR Solution Structure of the Precursor for Carnobacteriocin B2, an Antimicrobial Peptide from Carnobacterium piscicola. " Eur. J. Biochem. 271, 1748-1756 (2004).
Assembly members:
precarnobacteriocin B2, polymer, 64 residues, 6739 Da.
Natural source: Common Name: Carnobacterium piscicola Taxonomy ID: 2751 Superkingdom: Eubacteria Kingdom: not available Genus/species: Carnobacterium piscicola
Experimental source: Production method: recombinant technology Host organism: Esherichia coli Vector: pMAL-c
Entity Sequences (FASTA):
precarnobacteriocin B2: MNSVKELNVKEMKQLHGGVN
YGNGVSCSKTKCSVNWGQAF
QERYTAGINSFVSGVASGAG
SIGR
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 173 |
| 1H chemical shifts | 376 |
| 15N chemical shifts | 74 |
| coupling constants | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | preCbnB2 | 1 |
Entities:
Entity 1, preCbnB2 64 residues - 6739 Da.
| 1 | MET | ASN | SER | VAL | LYS | GLU | LEU | ASN | VAL | LYS | ||||
| 2 | GLU | MET | LYS | GLN | LEU | HIS | GLY | GLY | VAL | ASN | ||||
| 3 | TYR | GLY | ASN | GLY | VAL | SER | CYS | SER | LYS | THR | ||||
| 4 | LYS | CYS | SER | VAL | ASN | TRP | GLY | GLN | ALA | PHE | ||||
| 5 | GLN | GLU | ARG | TYR | THR | ALA | GLY | ILE | ASN | SER | ||||
| 6 | PHE | VAL | SER | GLY | VAL | ALA | SER | GLY | ALA | GLY | ||||
| 7 | SER | ILE | GLY | ARG |
Samples:
sample_1: precarnobacteriocin B2, [U-15N], 1.0 mM; DSS 0.025 mM; trifluoroethanol-d3, [U-99.9% D], 30%
sample_2: precarnobacteriocin B2, [U-13C; U-15N], 1.0 mM; DSS 0.025 mM; trifluoroethanol-d3, [U-99.9% D], 30%
condition_1: pH: 2.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | condition_1 |
Software:
NMRPipe v2.2 - Data processing
NMRView v5.0 - Data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts