BMRB Entry 6080
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6080
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Title: 1H, 15N, 13C resonance assignments of the human protein tyrosine phosphatase PRL-1 PubMed: 15213447
Deposition date: 2004-01-14 Original release date: 2008-09-18
Authors: Laurence, Jennifer; Hallenga, Klaas; Stauffacher, Cynthia
Citation: Laurence, Jennifer; Hallenga, Klaas; Stauffacher, Cynthia. "Letter to the Editor: 1H, 15N, 13C resonance assignments of the human protein tyrosine phosphatase PRL-1" J. Biomol. NMR 29, 417-418 (2004).
Assembly members:
PRL-1, polymer, 173 residues, 19815 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PRL-1: MARMNRPAPVEVTYKNMRFL
ITHNPTNATLNKFIEELKKY
GVTTIVRVCEATYDTTLVEK
EGIHVLDWPFDDGAPPSNQI
VDDWLSLVKIKFREEPGCCI
AVHCVAGLGRAPVLVALALI
EGGMKYEDAVQFIRQKRRGA
FNSKQLLYLEKYRPKMRLRF
KDSNGHRNNCCIQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 314 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 673 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PRL-1 monomer | 1 |
Entities:
Entity 1, PRL-1 monomer 173 residues - 19815 Da.
| 1 | MET | ALA | ARG | MET | ASN | ARG | PRO | ALA | PRO | VAL | ||||
| 2 | GLU | VAL | THR | TYR | LYS | ASN | MET | ARG | PHE | LEU | ||||
| 3 | ILE | THR | HIS | ASN | PRO | THR | ASN | ALA | THR | LEU | ||||
| 4 | ASN | LYS | PHE | ILE | GLU | GLU | LEU | LYS | LYS | TYR | ||||
| 5 | GLY | VAL | THR | THR | ILE | VAL | ARG | VAL | CYS | GLU | ||||
| 6 | ALA | THR | TYR | ASP | THR | THR | LEU | VAL | GLU | LYS | ||||
| 7 | GLU | GLY | ILE | HIS | VAL | LEU | ASP | TRP | PRO | PHE | ||||
| 8 | ASP | ASP | GLY | ALA | PRO | PRO | SER | ASN | GLN | ILE | ||||
| 9 | VAL | ASP | ASP | TRP | LEU | SER | LEU | VAL | LYS | ILE | ||||
| 10 | LYS | PHE | ARG | GLU | GLU | PRO | GLY | CYS | CYS | ILE | ||||
| 11 | ALA | VAL | HIS | CYS | VAL | ALA | GLY | LEU | GLY | ARG | ||||
| 12 | ALA | PRO | VAL | LEU | VAL | ALA | LEU | ALA | LEU | ILE | ||||
| 13 | GLU | GLY | GLY | MET | LYS | TYR | GLU | ASP | ALA | VAL | ||||
| 14 | GLN | PHE | ILE | ARG | GLN | LYS | ARG | ARG | GLY | ALA | ||||
| 15 | PHE | ASN | SER | LYS | GLN | LEU | LEU | TYR | LEU | GLU | ||||
| 16 | LYS | TYR | ARG | PRO | LYS | MET | ARG | LEU | ARG | PHE | ||||
| 17 | LYS | ASP | SER | ASN | GLY | HIS | ARG | ASN | ASN | CYS | ||||
| 18 | CYS | ILE | GLN |
Samples:
sample_1: PRL-1, [U-95% 13C; U-98% 15N], 1.3 – 2.0 mM
sample_2: PRL-1, [U-98% 15N], 1.5 – 2.0 mM
sample_3: PRL-1, [1H methyl only, 2H U-98%; U-95% 13C; U-98% 15N], 2.0 mM
Ex-cond_1: pH: 6.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CCONH | not available | not available | not available |
| HCCONH | not available | not available | not available |
| CCCONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
Software:
SPARKY v3 -
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts