BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6097

Title: Proton chemical shift assignments for human spliceosomal protein U1C zinc finger domain   PubMed: 15312772

Deposition date: 2004-02-10 Original release date: 2004-09-28

Authors: Muto, Yutaka; Pomeranz Krummel, Daniel; Oubridge, Chris; Hernandez, Helena; Robinson, Carol; Neuhaus, David; Nagai, Kiyoshi

Citation: Muto, Yutaka; Pomeranz Krummel, Daniel; Oubridge, Chris; Hernandez, Helena; Robinson, Carol; Neuhaus, David; Nagai, Kiyoshi. "The structure and biochemical properties of the human spliceosomal protein U1C"  J. Mol. Biol. 341, 185-198 (2004).

Assembly members:
U1C protein, polymer, 61 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3b

Entity Sequences (FASTA):
U1C protein: MPKFYCDYCDTYLTHDSPSV RKTHCSGRKHKENVKDYYQK WMEEQAQSLIDKTTAAFQQG K

Data sets:
Data typeCount
1H chemical shifts377

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U1C zinc finger domain1
2ZINC (II) ION2

Entities:

Entity 1, U1C zinc finger domain 61 residues - Formula weight is not available

1   METPROLYSPHETYRCYSASPTYRCYSASP
2   THRTYRLEUTHRHISASPSERPROSERVAL
3   ARGLYSTHRHISCYSSERGLYARGLYSHIS
4   LYSGLUASNVALLYSASPTYRTYRGLNLYS
5   TRPMETGLUGLUGLNALAGLNSERLEUILE
6   ASPLYSTHRTHRALAALAPHEGLNGLNGLY
7   LYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: U1C protein0.8 – 1.0 mM; potassium phosphate 20 mM; magnesium sulphate 100 mM; sodium bromide 400 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availableEx-cond_1
DQF-COSYsample_1not availableEx-cond_1
NOESYsample_1not availableEx-cond_1

Software:

xwin-nmr v3.0 - data processing

SPARKY - analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAE02052 BAE23273 BAE40776 BAG34895 BAI46833
EMBL CAA31037 CAA45354 CAA65542 CAG11833 CBN80717
GB AAA36618 AAB08894 AAH08243 AAH84206 AAH91442
PIR I56013
REF NP_001016598 NP_001069802 NP_001084130 NP_001158541 NP_001177132
SP B0JYS7 D3ZCL3 E1C6F0 E2RGI3 F6TFD9
TPG DAA16701
AlphaFold B0JYS7 D3ZCL3 E1C6F0 E2RGI3 F6TFD9