BMRB Entry 6098
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6098
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Title: Backbone 1H, 13C, and 15N chemical shift assignments for the amino terminal domain of the transcriptional cofactor PC4
Deposition date: 2004-02-13 Original release date: 2004-06-25
Authors: Jonker, Henry; Folkers, Gert; Wechselberger, Rainer; Boelens, Rolf; Kaptein, Rob
Citation: Jonker, Henry. "Structural and functional studies on transcriptional activation - Interactions of the cofactor PC4 and activator VP16" Thesis Utrecht University (2003).
Assembly members:
positive cofactor 4, polymer, 126 residues, 14264 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
positive cofactor 4: PKSKELVSSSSSGSDSDSEV
DKKLKRKKQVAPEKPVKKQK
TGETSRALSSSKQSSSSRDD
NMFQIGKMRYVSVRDFKGKV
LIDIREYWMDPEGEMKPGRK
GISLNPEQWSQLKEQISDID
DAVRKL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 492 |
| 1H chemical shifts | 217 |
| 15N chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PC4ntd, 1 | 1 |
| 2 | PC4ctd, 2 | 1 |
Entities:
Entity 1, PC4ntd, 1 126 residues - 14264 Da.
| 1 | PRO | LYS | SER | LYS | GLU | LEU | VAL | SER | SER | SER | ||||
| 2 | SER | SER | GLY | SER | ASP | SER | ASP | SER | GLU | VAL | ||||
| 3 | ASP | LYS | LYS | LEU | LYS | ARG | LYS | LYS | GLN | VAL | ||||
| 4 | ALA | PRO | GLU | LYS | PRO | VAL | LYS | LYS | GLN | LYS | ||||
| 5 | THR | GLY | GLU | THR | SER | ARG | ALA | LEU | SER | SER | ||||
| 6 | SER | LYS | GLN | SER | SER | SER | SER | ARG | ASP | ASP | ||||
| 7 | ASN | MET | PHE | GLN | ILE | GLY | LYS | MET | ARG | TYR | ||||
| 8 | VAL | SER | VAL | ARG | ASP | PHE | LYS | GLY | LYS | VAL | ||||
| 9 | LEU | ILE | ASP | ILE | ARG | GLU | TYR | TRP | MET | ASP | ||||
| 10 | PRO | GLU | GLY | GLU | MET | LYS | PRO | GLY | ARG | LYS | ||||
| 11 | GLY | ILE | SER | LEU | ASN | PRO | GLU | GLN | TRP | SER | ||||
| 12 | GLN | LEU | LYS | GLU | GLN | ILE | SER | ASP | ILE | ASP | ||||
| 13 | ASP | ALA | VAL | ARG | LYS | LEU |
Samples:
sample_PC4_298K_LS: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 50 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM
sample_PC4_305K_HS: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 400 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM
sample_PC4_P: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 400 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM
condition_PC4_298K_LS: pH: 5.6; temperature: 298 K
condition_PC4_305K_HS: pH: 5.6; temperature: 305 K
condition_PC4_P: pH: 5.6; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D (1H,15N)-HSQC | not available | not available | not available |
| 3D NOESY (1H,15N) HSQC | not available | not available | not available |
| 3D TOCSY (1H,15N) HSQC | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CA)CO | not available | not available | not available |
Software:
NMRPipe - processing
SPARKY v3 - analyses, assignment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92522 BAE00374 BAE87869 BAE91489 BAG10763 |
| EMBL | CAA56200 CAG33183 CAH92686 |
| GB | AAA20980 AAH09610 AAH10537 AAH18189 AAH22339 |
| REF | NP_001098877 NP_001126574 NP_001248140 NP_001272042 NP_006704 |
| SP | P53999 Q4R947 Q5R6D0 |
| TPG | DAA17835 |
| AlphaFold | P53999 Q4R947 Q5R6D0 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts