BMRB Entry 6112
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6112
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Title: Backbone 1H, 13C and 15N Chemical Shift Assignments for p27-KID in Solution PubMed: 15024385
Deposition date: 2004-02-23 Original release date: 2004-05-15
Authors: Lacy, Eilyn; Filippov, Igor; Lewis, William; Otieno, Steve; Xiao, Limin; Weiss, Sonja; Hengst, Ludger; Kriwacki, Richard
Citation: Lacy, Eilyn; Filippov, Igor; Lewis, William; Otieno, Steve; Xiao, Limin; Weiss, Sonja; Hengst, Ludger; Kriwacki, Richard. "p27 binds cyclin-CDK complexes through a sequential mechanism involving binding-induced protein folding" Nat. Struct. Mol. Biol. 11, 358-364 (2004).
Assembly members:
Cyclin dependent kinase inhibitor 1B, polymer, 88 residues, 10406 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia Coli Vector: pET28a
Entity Sequences (FASTA):
Cyclin dependent kinase inhibitor 1B: GSHMEHPKPSACRNLFGPVD
HEELTRDLEKHCRDMEEASQ
RKWNFDFQNHKPLEGKYEWQ
EVEKGSLPEFYYRPPRPPKG
ACKVPAQE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 74 |
| 13C chemical shifts | 82 |
| 15N chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p27-KID | 1 |
Entities:
Entity 1, p27-KID 88 residues - 10406 Da.
| 1 | GLY | SER | HIS | MET | GLU | HIS | PRO | LYS | PRO | SER | ||||
| 2 | ALA | CYS | ARG | ASN | LEU | PHE | GLY | PRO | VAL | ASP | ||||
| 3 | HIS | GLU | GLU | LEU | THR | ARG | ASP | LEU | GLU | LYS | ||||
| 4 | HIS | CYS | ARG | ASP | MET | GLU | GLU | ALA | SER | GLN | ||||
| 5 | ARG | LYS | TRP | ASN | PHE | ASP | PHE | GLN | ASN | HIS | ||||
| 6 | LYS | PRO | LEU | GLU | GLY | LYS | TYR | GLU | TRP | GLN | ||||
| 7 | GLU | VAL | GLU | LYS | GLY | SER | LEU | PRO | GLU | PHE | ||||
| 8 | TYR | TYR | ARG | PRO | PRO | ARG | PRO | PRO | LYS | GLY | ||||
| 9 | ALA | CYS | LYS | VAL | PRO | ALA | GLN | GLU |
Samples:
p27KID: Cyclin dependent kinase inhibitor 1B, [U-2H; U-13C; U-15N], 1.0 mM
Cond_set_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CT-HNCA | p27KID | not available | Cond_set_1 |
| CT-HN(CO)CA | p27KID | not available | Cond_set_1 |
| CT-HN(CA)CB | p27KID | not available | Cond_set_1 |
| CT-HN(COCA)CB | p27KID | not available | Cond_set_1 |
Software:
Felix -
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG35368 BAG60584 BAG70105 BAG70237 BAG73064 |
| EMBL | CAA59284 CAE82383 CAG33680 CAL37442 CAL38417 |
| GB | AAA20240 AAF69497 AAH01971 AAL78041 AAV38358 |
| REF | NP_004055 XP_002822998 XP_003808898 XP_004052799 XP_005680873 |
| SP | P46527 |
| AlphaFold | P46527 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts