BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6132

Title: Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem peroxiredoxin   PubMed: 15452441

Deposition date: 2004-03-04 Original release date: 2004-09-30

Authors: Bouillac, Sandrine; Rouhier, Nicolas; Tsan, Pascale; Jacquot, Jean-Pierre; Lancelin, Jean-Marc

Citation: Bouillac, Sandrine; Rouhier, Nicolas; Tsan, Pascale; Jacquot, Jean-Pierre; Lancelin, Jean-Marc. "Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin"  J. Biomol. NMR 30, 105-106 (2004).

Assembly members:
peroxiredoxin, polymer, 162 residues, 17408 Da.

Natural source:   Common Name: poplar   Taxonomy ID: 3689   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus trichocarpa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3d

Entity Sequences (FASTA):
peroxiredoxin: MAPIAVGDVLPDGKLAYFDE QDQLQEVSVHSLVAGKKVIL FGVPGAFTPTCSLKHVPGFI EKAGELKSKGVTEILCISVN DPFVMKAWAKSYPENKHVKF LADGSATYTHALGLELDLQG KGLGTRSRRFALLVDDLKVK AANIEGGGEFTVSSAEDILK DL

Data sets:
Data typeCount
13C chemical shifts604
15N chemical shifts151
1H chemical shifts895

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prx subunit 11
2prx subunit 21

Entities:

Entity 1, prx subunit 1 162 residues - 17408 Da.

1   METALAPROILEALAVALGLYASPVALLEU
2   PROASPGLYLYSLEUALATYRPHEASPGLU
3   GLNASPGLNLEUGLNGLUVALSERVALHIS
4   SERLEUVALALAGLYLYSLYSVALILELEU
5   PHEGLYVALPROGLYALAPHETHRPROTHR
6   CYSSERLEULYSHISVALPROGLYPHEILE
7   GLULYSALAGLYGLULEULYSSERLYSGLY
8   VALTHRGLUILELEUCYSILESERVALASN
9   ASPPROPHEVALMETLYSALATRPALALYS
10   SERTYRPROGLUASNLYSHISVALLYSPHE
11   LEUALAASPGLYSERALATHRTYRTHRHIS
12   ALALEUGLYLEUGLULEUASPLEUGLNGLY
13   LYSGLYLEUGLYTHRARGSERARGARGPHE
14   ALALEULEUVALASPASPLEULYSVALLYS
15   ALAALAASNILEGLUGLYGLYGLYGLUPHE
16   THRVALSERSERALAGLUASPILELEULYS
17   ASPLEU

Samples:

sample_1: peroxiredoxin, [U-100% 15N; U-100% 13C; 50% 2H], 1.5 mM; potassium phosphate 50 mM

sample_2: peroxiredoxin, [U-100% 15N], 1.1 mM; potassium phosphate 50 mM

Ex-cond: pH: 7.2; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availableEx-cond
1H-15N NOESY-HSQCnot availablenot availableEx-cond
HNCOnot availablenot availableEx-cond
HN(CA)COnot availablenot availableEx-cond
HNCAnot availablenot availableEx-cond
HN(CO)CAnot availablenot availableEx-cond
HN(CA)CBnot availablenot availableEx-cond
HN(COCA)CBnot availablenot availableEx-cond
(H)C(CCO)NH-TOCSYnot availablenot availableEx-cond
H(CCO)NH-TOCSYnot availablenot availableEx-cond
1H-1H TOCSYnot availablenot availableEx-cond
1H-1H NOESYnot availablenot availableEx-cond

Software:

NMRPipe - processing

PIPP - assignment

NMR spectrometers:

  • Varian Inova 800 MHz
  • Varian Inova 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
GenBank AAL90751 ABK94117

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts