BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6143

Title: 1H and 15N assignments for human apolipoprotein C-II in DPC micelles   PubMed: 15209504

Deposition date: 2004-03-14 Original release date: 2005-03-14

Authors: MacRaild, Christopher; Howlett, Geoffrey; Gooley, Paul

Citation: MacRaild, Christopher; Howlett, Geoffrey; Gooley, Paul. "The Structure and Interactions of Human Apolipoprotein C-II in Dodecyl Phosphocholine"  Biochemistry 43, 8084-8093 (2004).

Assembly members:
apoC-II, polymer, 79 residues, 8916 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
apoC-II: TQQPQQDEMPSPTFLTQVKE SLSSYWESAKTAAQNLYEKT YLPAVDEKLRDLYSKSTAAM STYTGIFTDQVLSVLKGEE

Data sets:
Data typeCount
1H chemical shifts273
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apolipoprotein C-II1

Entities:

Entity 1, apolipoprotein C-II 79 residues - 8916 Da.

1   THRGLNGLNPROGLNGLNASPGLUMETPRO
2   SERPROTHRPHELEUTHRGLNVALLYSGLU
3   SERLEUSERSERTYRTRPGLUSERALALYS
4   THRALAALAGLNASNLEUTYRGLULYSTHR
5   TYRLEUPROALAVALASPGLULYSLEUARG
6   ASPLEUTYRSERLYSSERTHRALAALAMET
7   SERTHRTYRTHRGLYILEPHETHRASPGLN
8   VALLEUSERVALLEULYSGLYGLUGLU

Samples:

sample_1: apoC-II, [U-15N], 1.8 mM; DPC 85 mM; sodium acetate 20 mM; sodium azide 0.1 mM

Ex-cond_1: pH: 5.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HMQCsample_1not availableEx-cond_1
1H-15N NOESYsample_1not availableEx-cond_1
1H-15C TOCSYsample_1not availableEx-cond_1
HNHAsample_1not availableEx-cond_1
HNHBsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 5534
PDB
EMBL CAA25234 CAA28798
GB AAA51743 AAA98743 AAB21042 AAB59380 AAH05348
PRF 1303321A
REF NP_000474 NP_001230828 XP_008969010
SP P02655
AlphaFold P02655

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts