BMRB Entry 6199

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6199

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Title:
Backbone and side chain 1H, 13C, and 15N chemical shift assignments for 5,10-methenyltetrahydrofolate synthetase
Deposition date:
2004-04-28
Original release date:
2007-08-24
Authors:
Cort, John; Kennedy, Michael; Montelione, Gaetano
Citation:

Citation: Cort, John; Kennedy, Michael; Montelione, Gaetano; Chiang, Yiwen; Acton, Thomas; Wu, M.. "Structure of 5,10 methenyltetrahydrofolate synthetase from Aquifex aeolicus"  .

Assembly members:

Assembly members:
5,10-methenyltetrahydrofolate, polymer, 194 residues, 22318 Da.

Natural source:

Natural source:   Common Name: Aquifex aeolicus   Taxonomy ID: 63363   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Aquifex aeolicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5,10-methenyltetrahydrofolate: MLKSELRKKVLHKRINLSEE ERRRLSEKVISNLKSLPEFK KSKKVALYCPIKGEVDLTPL FPEVLKEKELILPKVEGNEI SLYRVHSPACLGVGAFGIME PVEGERVNPEDVDFIAVPGV AFDLEGYRLGFGKGYYDRLL KRVKGLKVGVAYSFQVFERL PRDAWDIPVDVLVTEKNVRR LRDGRSLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts1142
13C chemical shifts737
15N chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15,10 methenyltetrahydrofolate synthetase1

Entities:

Entity 1, 5,10 methenyltetrahydrofolate synthetase 194 residues - 22318 Da.

1   METLEULYSSERGLULEUARGLYSLYSVAL
2   LEUHISLYSARGILEASNLEUSERGLUGLU
3   GLUARGARGARGLEUSERGLULYSVALILE
4   SERASNLEULYSSERLEUPROGLUPHELYS
5   LYSSERLYSLYSVALALALEUTYRCYSPRO
6   ILELYSGLYGLUVALASPLEUTHRPROLEU
7   PHEPROGLUVALLEULYSGLULYSGLULEU
8   ILELEUPROLYSVALGLUGLYASNGLUILE
9   SERLEUTYRARGVALHISSERPROALACYS
10   LEUGLYVALGLYALAPHEGLYILEMETGLU
11   PROVALGLUGLYGLUARGVALASNPROGLU
12   ASPVALASPPHEILEALAVALPROGLYVAL
13   ALAPHEASPLEUGLUGLYTYRARGLEUGLY
14   PHEGLYLYSGLYTYRTYRASPARGLEULEU
15   LYSARGVALLYSGLYLEULYSVALGLYVAL
16   ALATYRSERPHEGLNVALPHEGLUARGLEU
17   PROARGASPALATRPASPILEPROVALASP
18   VALLEUVALTHRGLULYSASNVALARGARG
19   LEUARGASPGLYARGSERLEUGLUHISHIS
20   HISHISHISHIS

Samples:

sample_1: 5,10-methenyltetrahydrofolate, [U-13C; U-15N], 1 mM; MES (2-morpholinoethanesulfonic acid) 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_2: 5,10-methenyltetrahydrofolate, [U-13C; U-15N], 1 mM; MES (2-morpholinoethanesulfonic acid) 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; D2O 100%

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D N15 NOESY-HSQCsample_1not availableEx-cond_1
3D C13 NOESY-HSQCsample_1not availableEx-cond_1
1H-15N-HSQCnot availablenot availableEx-cond_1
1H-13C-HSQCnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HN(CO)CAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCC-TOCSY-NNHnot availablenot availableEx-cond_1
CCC-TOCSY-NNHnot availablenot availableEx-cond_1
CBCACOCAHAnot availablenot availableEx-cond_1
HBCBCGCDHD-AROnot availablenot availableEx-cond_1
HBCBCGCDCEHE-AROnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
4D-13C-13C-HMQC-NOESY-HMQCnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 800 MHz
  • Varian Inova 750 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAC07587
REF NP_214187 WP_010881124

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks