BMRB Entry 6255
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6255
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Chemical Shift Assignments of Ribosomal Protein L16 from Thermus thermophilus HB8
Deposition date: 2004-06-27 Original release date: 2004-12-06
Authors: Nishimura, Mitsuhiro; Yoshida, Takuya; Shirouzu, Mikako; Terada, Takaho; Kuramitsu, Seiki; Yokoyama, Shigeyuki; Ohkubo, Tadayasu; Kobayashi, Yuji
Citation: Nishimura, Mitsuhiro; Yoshida, Takuya; Shirouzu, Mikako; Terada, Takaho; Kuramitsu, Seiki; Yokoyama, Shigeyuki; Ohkubo, Tadayasu; Kobayashi, Yuji. "Solution Structure of Ribosomal Protein L16 from Thermus thermophilus HB8" J. Mol. Biol. 344, 1369-1383 (2004).
Assembly members:
Ribosomal Protein L16, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermohilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET26b
Entity Sequences (FASTA):
Ribosomal Protein L16: MLMPRRMKYRKQQRGRLKGA
TKGGDYVAFGDYGLVALEPA
WITAQQIEAARVAMVRHFRR
GGKIFIRIFPDKPYTKKPLE
VRMGKGKGNVEGYVAVVKPG
RVMFEVAGVTEEQAMEALRI
AGHKLPIKTKIVRRDAYDEA
Q
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 960 |
| 13C chemical shifts | 624 |
| 15N chemical shifts | 134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ribosomal Protein L16 | 1 |
Entities:
Entity 1, Ribosomal Protein L16 141 residues - Formula weight is not available
| 1 | MET | LEU | MET | PRO | ARG | ARG | MET | LYS | TYR | ARG | ||||
| 2 | LYS | GLN | GLN | ARG | GLY | ARG | LEU | LYS | GLY | ALA | ||||
| 3 | THR | LYS | GLY | GLY | ASP | TYR | VAL | ALA | PHE | GLY | ||||
| 4 | ASP | TYR | GLY | LEU | VAL | ALA | LEU | GLU | PRO | ALA | ||||
| 5 | TRP | ILE | THR | ALA | GLN | GLN | ILE | GLU | ALA | ALA | ||||
| 6 | ARG | VAL | ALA | MET | VAL | ARG | HIS | PHE | ARG | ARG | ||||
| 7 | GLY | GLY | LYS | ILE | PHE | ILE | ARG | ILE | PHE | PRO | ||||
| 8 | ASP | LYS | PRO | TYR | THR | LYS | LYS | PRO | LEU | GLU | ||||
| 9 | VAL | ARG | MET | GLY | LYS | GLY | LYS | GLY | ASN | VAL | ||||
| 10 | GLU | GLY | TYR | VAL | ALA | VAL | VAL | LYS | PRO | GLY | ||||
| 11 | ARG | VAL | MET | PHE | GLU | VAL | ALA | GLY | VAL | THR | ||||
| 12 | GLU | GLU | GLN | ALA | MET | GLU | ALA | LEU | ARG | ILE | ||||
| 13 | ALA | GLY | HIS | LYS | LEU | PRO | ILE | LYS | THR | LYS | ||||
| 14 | ILE | VAL | ARG | ARG | ASP | ALA | TYR | ASP | GLU | ALA | ||||
| 15 | GLN |
Samples:
sample_1: Ribosomal Protein L16, [U-15N], 0.6 mM; Sodium acetate 10 mM; Sodium chloride 100 mM
sample_2: Ribosomal Protein L16, [U-13C; U-15N], 0.6 mM; Sodium acetate 10 mM; Sodium chloride 100 mM
sample_cond: pH: 5.4 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | not available | not available | sample_cond |
| 15N-separated NOESY | not available | not available | sample_cond |
| CBCA(CO)NH | not available | not available | sample_cond |
| CBCANH | not available | not available | sample_cond |
| HNCAHA | not available | not available | sample_cond |
| C(CO)NH | not available | not available | sample_cond |
| HCCH-TOCSY | not available | not available | sample_cond |
| HBHA(CO)NH | not available | not available | sample_cond |
| H(CCO)NH | not available | not available | sample_cond |
| HNCO | not available | not available | sample_cond |
| 13C-1H HSQC | not available | not available | sample_cond |
| 13C-separated NOESY | not available | not available | sample_cond |
Software:
NMRPipe - Data processing
SPARKY v3.106, Spectrum analysis - Data processing
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD71508 |
| GB | AAS81663 AEG34098 AFH38269 EED11193 EIA38546 |
| REF | WP_003043874 WP_008633417 WP_011173709 WP_011228846 WP_019550491 |
| SP | P60489 Q72I11 |
| AlphaFold | P60489 Q72I11 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts