BMRB Entry 6258
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6258
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Title: Backbone 1H, 13C, 15N and Cb chemical shift assignments for TolAIII in complex with g3pN1 PubMed: 15701516
Deposition date: 2004-06-30 Original release date: 2005-02-21
Authors: Deprez, Christophe; Lloubes, Roland; Gavioli, Marthe; Marion, Dominique; Guerlesquin, Francoise; Blanchard, Laurence
Citation: Deprez, Christophe; Lloubes, Roland; Gavioli, Marthe; Marion, Dominique; Guerlesquin, Francoise; Blanchard, Laurence. "Solution Structure of the E.coli TolA C-terminal Domain Reveals Conformational Changes upon Binding to the Phage g3p N-terminal Domain" J. Mol. Biol. 346, 1047-1057 (2005).
Assembly members:
TolA domain III, polymer, 106 residues, Formula weight is not available
g3pN1, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia E. coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
TolA domain III: AEFGNTKNNGASGADINNYA
GQIKSAIESKFYDASSYAGK
TCTLRIKLAPDGMLLDIKPE
GGDPALCQAALAAAKLAKIP
KPPSQAVYEVFKNAPLDFKP
HHHHHH
g3pN1: MKKLLFAIPLVVPFYSHSAE
TVESCLAKPHTENSFTNVWK
DDKTLDRYANYEGCLWNATG
VVVCTGDETQCHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 225 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TolAIII | 1 |
| 2 | g3pN1 | 2 |
Entities:
Entity 1, TolAIII 106 residues - Formula weight is not available
| 1 | ALA | GLU | PHE | GLY | ASN | THR | LYS | ASN | ASN | GLY | ||||
| 2 | ALA | SER | GLY | ALA | ASP | ILE | ASN | ASN | TYR | ALA | ||||
| 3 | GLY | GLN | ILE | LYS | SER | ALA | ILE | GLU | SER | LYS | ||||
| 4 | PHE | TYR | ASP | ALA | SER | SER | TYR | ALA | GLY | LYS | ||||
| 5 | THR | CYS | THR | LEU | ARG | ILE | LYS | LEU | ALA | PRO | ||||
| 6 | ASP | GLY | MET | LEU | LEU | ASP | ILE | LYS | PRO | GLU | ||||
| 7 | GLY | GLY | ASP | PRO | ALA | LEU | CYS | GLN | ALA | ALA | ||||
| 8 | LEU | ALA | ALA | ALA | LYS | LEU | ALA | LYS | ILE | PRO | ||||
| 9 | LYS | PRO | PRO | SER | GLN | ALA | VAL | TYR | GLU | VAL | ||||
| 10 | PHE | LYS | ASN | ALA | PRO | LEU | ASP | PHE | LYS | PRO | ||||
| 11 | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, g3pN1 77 residues - Formula weight is not available
| 1 | MET | LYS | LYS | LEU | LEU | PHE | ALA | ILE | PRO | LEU | ||||
| 2 | VAL | VAL | PRO | PHE | TYR | SER | HIS | SER | ALA | GLU | ||||
| 3 | THR | VAL | GLU | SER | CYS | LEU | ALA | LYS | PRO | HIS | ||||
| 4 | THR | GLU | ASN | SER | PHE | THR | ASN | VAL | TRP | LYS | ||||
| 5 | ASP | ASP | LYS | THR | LEU | ASP | ARG | TYR | ALA | ASN | ||||
| 6 | TYR | GLU | GLY | CYS | LEU | TRP | ASN | ALA | THR | GLY | ||||
| 7 | VAL | VAL | VAL | CYS | THR | GLY | ASP | GLU | THR | GLN | ||||
| 8 | CYS | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: TolA domain III, [U-13C; U-15N], 0.4 mM; g3pN1 0.6 mM; potassium phosphate buffer 30 mM; NaCl 30 mM
cond_1: ionic strength: 60 mM; pH: 6.8; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D DEPT-CBCA(CO)NH | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
Software:
FELIX -
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 4771 |
| PDB | |
| DBJ | BAA35405 BAB34197 BAF74208 BAF74218 |
| GenBank | AAA24683 AAC73833 AAG55075 AAN42202 AAN79291 AAA32215 AAA32309 AAF75609 AAM73889 AAM73901 |
| REF | AP_001377 NP_286467 NP_308801 NP_415267 NP_706495 NP_510891 |
| SWISS-PROT | P19934 P03661 P69168 P69169 |
| EMBL | CAA23862 CAA23872 |
| PIR | Z3BPF1 |
| PRF | 0812197D |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts