BMRB Entry 6267
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6267
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Title: 1H, 13C and 15N resonance assignments of the phosphorylated enzyme IIB (C31S) of the mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli
Deposition date: 2004-07-19 Original release date: 2005-01-07
Authors: Otten, Renee; Van Lune, Franciska; Dijkstra, Klaas; Scheek, Ruud
Citation: Otten, Renee; Van Lune, Franciska; Dijkstra, Klaas; Scheek, Ruud. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the phosphorylated enzyme IIB of the mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli" J. Biomol. NMR 30, 461-462 (2004).
Assembly members:
phospho-IIB-mannitol, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
phospho-IIB-mannitol: GSHMSHVRKIIVASDAGMGS
SAMGAGVLRKKIQDAGLSQI
SVTNSAINNLPPDVDLVITH
RDLTERAMRQVPQAQHISLT
NFLDSGLYTSLTERLVAAQR
HTANEEKVKDSLKDSFD
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 737 |
13C chemical shifts | 453 |
15N chemical shifts | 120 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | phospho-IIB-mannitol | 1 |
Entities:
Entity 1, phospho-IIB-mannitol 117 residues - Formula weight is not available
1 | GLY | SER | HIS | MET | SER | HIS | VAL | ARG | LYS | ILE | ||||
2 | ILE | VAL | ALA | SER | ASP | ALA | GLY | MET | GLY | SER | ||||
3 | SER | ALA | MET | GLY | ALA | GLY | VAL | LEU | ARG | LYS | ||||
4 | LYS | ILE | GLN | ASP | ALA | GLY | LEU | SER | GLN | ILE | ||||
5 | SER | VAL | THR | ASN | SER | ALA | ILE | ASN | ASN | LEU | ||||
6 | PRO | PRO | ASP | VAL | ASP | LEU | VAL | ILE | THR | HIS | ||||
7 | ARG | ASP | LEU | THR | GLU | ARG | ALA | MET | ARG | GLN | ||||
8 | VAL | PRO | GLN | ALA | GLN | HIS | ILE | SER | LEU | THR | ||||
9 | ASN | PHE | LEU | ASP | SER | GLY | LEU | TYR | THR | SER | ||||
10 | LEU | THR | GLU | ARG | LEU | VAL | ALA | ALA | GLN | ARG | ||||
11 | HIS | THR | ALA | ASN | GLU | GLU | LYS | VAL | LYS | ASP | ||||
12 | SER | LEU | LYS | ASP | SER | PHE | ASP |
Samples:
sample: phospho-IIB-mannitol, [U-13C; U-15N], 2.0 mM; Tris-Acetate buffer 20 mM; DTT 3 mM; NaN3 1 mM; D2O 5%; EDTA 1 mM
Ex-cond_1: pH: 6.2; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
[15N,1H]-cpmg-HSQC | sample | not available | Ex-cond_1 |
HNCA | sample | not available | Ex-cond_1 |
HN(CO)CA | sample | not available | Ex-cond_1 |
HN(CA)CO | sample | not available | Ex-cond_1 |
HNCO | sample | not available | Ex-cond_1 |
HNCACB | sample | not available | Ex-cond_1 |
CBCA(CO)NH | sample | not available | Ex-cond_1 |
COCAH | sample | not available | Ex-cond_1 |
HC(C)H-TOCSY | sample | not available | Ex-cond_1 |
HBHA(CBCACO)NH | sample | not available | Ex-cond_1 |
CBCGCDCEHE | sample | not available | Ex-cond_1 |
CBCGCDHD | sample | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity INOVA 600 MHz
Related Database Links:
PDB | |
DBJ | BAE77694 BAG79401 BAJ45339 BAL40192 |
EMBL | CAA24748 CAQ33922 CAQ91062 CAR00566 CAV00578 |
GB | AAB18576 AAC76623 ABV08013 ACB04648 ACR63207 |
REF | NP_418056 WP_000093235 WP_000093236 WP_000093240 WP_000093243 |
SP | P00550 |
AlphaFold | P00550 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
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or all simulated shifts