BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6270

Title: 1H, 13C, and 15N Chemical Shift Assignments for chPrP(25-242)   PubMed: 15459548

Deposition date: 2004-07-22 Original release date: 2004-10-18

Authors: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt

Citation: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt. "Letters to the Editor: NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242)"  J. Biomol. NMR 30, 97-97 (2004).

Assembly members:
chicken prion protein, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
chicken prion protein: GSKKGKGKPSGGGWGAGSHR QPSYPRQPGYPHNPGYPHNP GYPHNPGYPHNPGYPQNPGY PHNPGYPGWGQGYNPSSGGS YHNQKPWKPPKTNFKHVAGA AAAGAVV

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts82
1H chemical shifts476

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chPrP (25-242)1

Entities:

Entity 1, chPrP (25-242) 107 residues - Formula weight is not available

1   GLYSERLYSLYSGLYLYSGLYLYSPROSER
2   GLYGLYGLYTRPGLYALAGLYSERHISARG
3   GLNPROSERTYRPROARGGLNPROGLYTYR
4   PROHISASNPROGLYTYRPROHISASNPRO
5   GLYTYRPROHISASNPROGLYTYRPROHIS
6   ASNPROGLYTYRPROGLNASNPROGLYTYR
7   PROHISASNPROGLYTYRPROGLYTRPGLY
8   GLNGLYTYRASNPROSERSERGLYGLYSER
9   TYRHISASNGLNLYSPROTRPLYSPROPRO
10   LYSTHRASNPHELYSHISVALALAGLYALA
11   ALAALAALAGLYALAVALVAL

Samples:

sample_1: chicken prion protein, [U-30% 13C; U-30% 15N], 1.0 mM; sodium acetate 10 mM

Ex-cond_1: ionic strength: 10 mM; pH: 4.3; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-13N NOESY aliphatic, aromaticnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

GB AAA49041 AAF73436 AAF73437 AAR21236

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts