BMRB Entry 6278
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6278
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Sed5p PubMed: 11879635
Deposition date: 2004-07-28 Original release date: 2004-09-28
Authors: Chen, Xiaocheng; Rizo, Josep
Citation: Yamaguchi, Tomohiro; Dulubova, Irina; Min, Sang-Won; Chen, Xiaocheng; Rizo, Josep; Sudhof, Thomas. "Sly1 Binds to Golgi and ER Syntaxins via a Conserved N-Terminal Peptide Motif" Dev. Cell 2, 295-305 (2002).
Assembly members:
Sed5p, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sed5p: KSVSEFQKKASGIAHEISST
AQLLSKLAVLAKRKPMFNDN
PVEIAELSFLIKRKIYAIEQ
SLVQLSQLKKTDVNGNTSNQ
SSKQPSAVQHSKNVVNLLNT
QMKNISGSFKDVLEERQRLE
MANKDRW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 341 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Yeast Sed5p | 1 |
Entities:
Entity 1, Yeast Sed5p 127 residues - Formula weight is not available
| 1 | LYS | SER | VAL | SER | GLU | PHE | GLN | LYS | LYS | ALA | ||||
| 2 | SER | GLY | ILE | ALA | HIS | GLU | ILE | SER | SER | THR | ||||
| 3 | ALA | GLN | LEU | LEU | SER | LYS | LEU | ALA | VAL | LEU | ||||
| 4 | ALA | LYS | ARG | LYS | PRO | MET | PHE | ASN | ASP | ASN | ||||
| 5 | PRO | VAL | GLU | ILE | ALA | GLU | LEU | SER | PHE | LEU | ||||
| 6 | ILE | LYS | ARG | LYS | ILE | TYR | ALA | ILE | GLU | GLN | ||||
| 7 | SER | LEU | VAL | GLN | LEU | SER | GLN | LEU | LYS | LYS | ||||
| 8 | THR | ASP | VAL | ASN | GLY | ASN | THR | SER | ASN | GLN | ||||
| 9 | SER | SER | LYS | GLN | PRO | SER | ALA | VAL | GLN | HIS | ||||
| 10 | SER | LYS | ASN | VAL | VAL | ASN | LEU | LEU | ASN | THR | ||||
| 11 | GLN | MET | LYS | ASN | ILE | SER | GLY | SER | PHE | LYS | ||||
| 12 | ASP | VAL | LEU | GLU | GLU | ARG | GLN | ARG | LEU | GLU | ||||
| 13 | MET | ALA | ASN | LYS | ASP | ARG | TRP |
Samples:
sample_1: Sed5p, [U-13C; U-15N], 0.5 mM
Ex-cond_1: pH: 6.5; temperature: 302 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
Software:
NMRView v4.12 - analyze NMR data
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| DBJ | GAA24913 |
| EMBL | CAA47390 CAA97549 CAY81264 |
| GB | AHY78432 EDN59573 EDV09334 EDZ70730 EEU04509 |
| REF | NP_013126 |
| SP | Q01590 |
| TPG | DAA09344 |
| AlphaFold | Q01590 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts