BMRB Entry 6297
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6297
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Title: Solution structure of the RWD domain of the mouse GCN2 protein PubMed: 15273307
Deposition date: 2004-08-26 Original release date: 2005-01-24
Authors: Nameki, Nobukazu; Yoneyama, Misao; Koshiba, Seizo; Tochio, Naoya; Inoue, Makoto; Seki, Eiko; Matsuda, Takayoshi; Tomo, Yasuko; Harada, Takushi; Saito, Kohei; Kobayashi, Naohiro; Yabuki, Takashi; Aoki, Masaaki; Nunokawa, Emi; Matsuda, Natsuko; Sakagami, Noriko; Terada, Takaho; Shirouzu, Mikako; Yoshida, Mayumi; Hirota, Hiroshi; Osanai, Takashi; Tanaka, Akiko; Arakawa, Takahiro; Carninci, Piero; Kawai, Jun; Hayashizaki, Yoshihide; Kinoshita, Kengo; Guntert, Peter; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation: Nameki, Nobukazu; Yoneyama, Misao; Koshiba, Seizo; Tochio, Naoya; Inoue, Makoto; Seki, Eiko; Matsuda, Takayoshi; Tomo, Yasuko; Harada, Takushi; Saito, Kohei; Kobayashi, Naohiro; Yabuki, Takashi; Aoki, Masaaki; Nunokawa, Emi; Matsuda, Natsuko; Sakagami, Noriko; Terada, Takaho; Shirouzu, Mikako; Yoshida, Mayumi; Hirota, Hiroshi; Osanai, Takashi; Tanaka, Akiko; Arakawa, Takahiro; Carninci, Piero; Kawai, Jun; Hayashizaki, Yoshihide; Kinoshita, Kengo; Guntert, Peter; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structure of the RWD domain of the mouse GCN2 protein" Protein Sci. 13, 2089-2100 (2004).
Assembly members:
RWD domain, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: P021021-28
Entity Sequences (FASTA):
RWD domain: GSSGSSGMESYSQRQDHELQ
ALEAIYGSDFQDLRPDARGR
VREPPEINLVLYPQGLAGEE
VYVQVELRVKCPPTYPDVVP
EIDLKNAKGLSNESVNLLKS
HLEELAKKQCGEVMIFELAH
HVQSFLSEHNKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 575 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 933 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GCN2, RWD_domain | 1 |
Entities:
Entity 1, GCN2, RWD_domain 137 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | GLU | SER | ||||
| 2 | TYR | SER | GLN | ARG | GLN | ASP | HIS | GLU | LEU | GLN | ||||
| 3 | ALA | LEU | GLU | ALA | ILE | TYR | GLY | SER | ASP | PHE | ||||
| 4 | GLN | ASP | LEU | ARG | PRO | ASP | ALA | ARG | GLY | ARG | ||||
| 5 | VAL | ARG | GLU | PRO | PRO | GLU | ILE | ASN | LEU | VAL | ||||
| 6 | LEU | TYR | PRO | GLN | GLY | LEU | ALA | GLY | GLU | GLU | ||||
| 7 | VAL | TYR | VAL | GLN | VAL | GLU | LEU | ARG | VAL | LYS | ||||
| 8 | CYS | PRO | PRO | THR | TYR | PRO | ASP | VAL | VAL | PRO | ||||
| 9 | GLU | ILE | ASP | LEU | LYS | ASN | ALA | LYS | GLY | LEU | ||||
| 10 | SER | ASN | GLU | SER | VAL | ASN | LEU | LEU | LYS | SER | ||||
| 11 | HIS | LEU | GLU | GLU | LEU | ALA | LYS | LYS | GLN | CYS | ||||
| 12 | GLY | GLU | VAL | MET | ILE | PHE | GLU | LEU | ALA | HIS | ||||
| 13 | HIS | VAL | GLN | SER | PHE | LEU | SER | GLU | HIS | ASN | ||||
| 14 | LYS | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: RWD domain, [U-15N; U-13C], 1.0 mM; dTris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D20 10%; H20 90%
sample_conditions: ionic strength: 120 mM; pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | sample_conditions |
| 1H13C HSQC | sample_1 | not available | sample_conditions |
| HNCO | sample_1 | not available | sample_conditions |
| HNCACO | sample_1 | not available | sample_conditions |
| HNCA | sample_1 | not available | sample_conditions |
| HNCACA | sample_1 | not available | sample_conditions |
| HNCACB | sample_1 | not available | sample_conditions |
| CBCACONH | sample_1 | not available | sample_conditions |
| HAHBNH | sample_1 | not available | sample_conditions |
| CCCONNH | sample_1 | not available | sample_conditions |
| HCCCONNH | sample_1 | not available | sample_conditions |
| HCCHCOSY | sample_1 | not available | sample_conditions |
| HCCHTOCSY | sample_1 | not available | sample_conditions |
| 15N NOESY | sample_1 | not available | sample_conditions |
| 13C NOESY | sample_1 | not available | sample_conditions |
Software:
NMRPipe v20020425 - processing
NMR spectrometers:
- Bruker AVANCE 700 MHz
- Bruker AVANCE 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts