BMRB Entry 6305
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6305
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Title: Mainchain proton, carbon and nitrogen chemical shifts for the C-domain of human RanGAP1 PubMed: 15355965
Deposition date: 2004-08-27 Original release date: 2004-11-29
Authors: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence
Citation: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence. "Structural and Dynamic Independence of Isopeptide-linked RanGAP1 and SUMO-1" J. Biol. Chem. 279, 49131-49137 (2004).
Assembly members:
RanGAP1, polymer, 172 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Animalia Genus/species: Homo sapian
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
RanGAP1: GSHMGEPAPVLSSPPPADVS
TFLAFPSPEKLLRLGPKSSV
LIAQQTDTSDPEKVVSAFLK
VSSVFKDEATVTMAVQDAVD
ALMQKAFNSSSFNSNTFLTR
LLVHMGLLKSEDKVKAIANL
YGPLMALNHMVQQDYFPKAL
APLLLAFVTKPNSALESCSF
ARHSLLQTLYKV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 459 |
| 15N chemical shifts | 152 |
| 1H chemical shifts | 152 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RanGAP1 C-domain | 1 |
Entities:
Entity 1, RanGAP1 C-domain 172 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLY | GLU | PRO | ALA | PRO | VAL | ||||
| 2 | LEU | SER | SER | PRO | PRO | PRO | ALA | ASP | VAL | SER | ||||
| 3 | THR | PHE | LEU | ALA | PHE | PRO | SER | PRO | GLU | LYS | ||||
| 4 | LEU | LEU | ARG | LEU | GLY | PRO | LYS | SER | SER | VAL | ||||
| 5 | LEU | ILE | ALA | GLN | GLN | THR | ASP | THR | SER | ASP | ||||
| 6 | PRO | GLU | LYS | VAL | VAL | SER | ALA | PHE | LEU | LYS | ||||
| 7 | VAL | SER | SER | VAL | PHE | LYS | ASP | GLU | ALA | THR | ||||
| 8 | VAL | THR | MET | ALA | VAL | GLN | ASP | ALA | VAL | ASP | ||||
| 9 | ALA | LEU | MET | GLN | LYS | ALA | PHE | ASN | SER | SER | ||||
| 10 | SER | PHE | ASN | SER | ASN | THR | PHE | LEU | THR | ARG | ||||
| 11 | LEU | LEU | VAL | HIS | MET | GLY | LEU | LEU | LYS | SER | ||||
| 12 | GLU | ASP | LYS | VAL | LYS | ALA | ILE | ALA | ASN | LEU | ||||
| 13 | TYR | GLY | PRO | LEU | MET | ALA | LEU | ASN | HIS | MET | ||||
| 14 | VAL | GLN | GLN | ASP | TYR | PHE | PRO | LYS | ALA | LEU | ||||
| 15 | ALA | PRO | LEU | LEU | LEU | ALA | PHE | VAL | THR | LYS | ||||
| 16 | PRO | ASN | SER | ALA | LEU | GLU | SER | CYS | SER | PHE | ||||
| 17 | ALA | ARG | HIS | SER | LEU | LEU | GLN | THR | LEU | TYR | ||||
| 18 | LYS | VAL |
Samples:
sample: RanGAP1; KCl 100 mM; KPhos 10 mM; DTT 2 mM; D2O 10%
sample_conditions: pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| 15N-editted NOESY-HSQC | not available | not available | not available |
| 15N-editted TOCSY-HSQC | not available | not available | not available |
Software:
NMRpipe -
NMRView -
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 6306 |
| PDB | |
| DBJ | BAB47464 BAG10161 BAG63578 BAG64552 |
| EMBL | CAA57714 CAG30443 CAK54588 CAK54887 |
| GB | AAH04990 AAH14044 AAH41396 AIC55014 EAW60420 |
| REF | NP_001265580 NP_002874 XP_005261752 XP_005261753 XP_006724352 |
| SP | P46060 |
| AlphaFold | P46060 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts