BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6354

Title: 1H, 13C and 15N resonance assignments and 15N-1H residual dipolar couplings for NECAP1 protein   PubMed: 17762867

Deposition date: 2004-10-13 Original release date: 2008-07-01

Authors: Denisov, Alexei; Gehring, Kalle

Citation: Ritter, Brigitte; Denisov, Alexey; Philie, Jacynthe; Allaire, Patrick; Legendre-Guillemin, Valerie; Zylbergold, Peter; Gehring, Kalle; McPherson, Peter. "Solution structure of NECAP1 protein"  EMBO J. 26, 4066-4077 (2007).

Assembly members:
NECAP1 protein, polymer, 133 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):
NECAP1 protein: MAAELEYESVLCVKPDVSVY RIPPRASNRGYRASDWKLDQ PDWTGRLRITSKGKIAYIKL EDKVSGELFAQAPVEQYPGI AVETVTDSSRYFVIRIQDGT GRSAFIGIGFTDRGDAFDFN VSLQDHFKWVKQE

Data sets:
Data typeCount
1H chemical shifts645
13C chemical shifts498
15N chemical shifts124
residual dipolar couplings121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NECAP1 monomer1

Entities:

Entity 1, NECAP1 monomer 133 residues - Formula weight is not available

1   METALAALAGLULEUGLUTYRGLUSERVAL
2   LEUCYSVALLYSPROASPVALSERVALTYR
3   ARGILEPROPROARGALASERASNARGGLY
4   TYRARGALASERASPTRPLYSLEUASPGLN
5   PROASPTRPTHRGLYARGLEUARGILETHR
6   SERLYSGLYLYSILEALATYRILELYSLEU
7   GLUASPLYSVALSERGLYGLULEUPHEALA
8   GLNALAPROVALGLUGLNTYRPROGLYILE
9   ALAVALGLUTHRVALTHRASPSERSERARG
10   TYRPHEVALILEARGILEGLNASPGLYTHR
11   GLYARGSERALAPHEILEGLYILEGLYPHE
12   THRASPARGGLYASPALAPHEASPPHEASN
13   VALSERLEUGLNASPHISPHELYSTRPVAL
14   LYSGLNGLU

Samples:

sample_1: NECAP1 protein, [U-99% 13C; U-99% 15N], 1.4 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_2: NECAP1 protein, [U-99% 15N], 1.4 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_3: NECAP1 protein, [U-99% 15N], 0.6 mM; sodium phosphate buffer 25 mM; NaCl 75 mM; Pf1 phage 8.0 mg/mL

Ex-cond_1: pH: 7.2; temperature: 303 K; ionic strength: 0.10 M

Experiments:

NameSampleSample stateSample conditions
IPAP-HSQCnot availablenot availableEx-cond_1

Software:

XWINNMR v3.5 - collection, processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

tpg|DAA01433.1| DAA01433
SWISS-PROT Q9CR95 Q8NC96 Q5R630 P69682
REF XP_001113344 XP_001113313 NP_080543 NP_056324 NP_001025090
GenBank AAI20842 AAI19134 AAI10877 AAH97496 AAH11466
EMBL CAH92786 CAH92713
DBJ BAC11296 BAC11264 BAC11250 BAB23915 BAB23577
PDB

Download HSQC peak lists in one of the following formats:
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