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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6357
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Julien, Olivier; Savard, Pierre-Yves; Gagne, Stephane. "Backbone Resonance Assignments of Wild-Type TEM-1 Beta-lactamase from E. coli" The BMRB entry is the only known published source for the data. (2004).
Assembly members:
TEM-1 beta-lactamase, polymer, 263 residues, 28907 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet24
Entity Sequences (FASTA):
TEM-1 beta-lactamase: HPETLVKVKDAEDQLGARVG
YIELDLNSGKILESFRPEER
FPMMSTFKVLLCGAVLSRVD
AGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSA
AITMSDNTAANLLLTTIGGP
KELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAA
MATTLRKLLTGELLTLASRQ
QLIDWMEADKVAGPLLRSAL
PAGWFIADKSGAGERGSRGI
IAALGPDGKPSRIVVIYTTG
SQATMDERNRQIAEIGASLI
KHW
Data type | Count |
1H chemical shifts | 248 |
13C chemical shifts | 727 |
15N chemical shifts | 248 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TEM-1 | 1 |
Entity 1, TEM-1 263 residues - 28907 Da.
1 | HIS | PRO | GLU | THR | LEU | VAL | LYS | VAL | LYS | ASP | ||||
2 | ALA | GLU | ASP | GLN | LEU | GLY | ALA | ARG | VAL | GLY | ||||
3 | TYR | ILE | GLU | LEU | ASP | LEU | ASN | SER | GLY | LYS | ||||
4 | ILE | LEU | GLU | SER | PHE | ARG | PRO | GLU | GLU | ARG | ||||
5 | PHE | PRO | MET | MET | SER | THR | PHE | LYS | VAL | LEU | ||||
6 | LEU | CYS | GLY | ALA | VAL | LEU | SER | ARG | VAL | ASP | ||||
7 | ALA | GLY | GLN | GLU | GLN | LEU | GLY | ARG | ARG | ILE | ||||
8 | HIS | TYR | SER | GLN | ASN | ASP | LEU | VAL | GLU | TYR | ||||
9 | SER | PRO | VAL | THR | GLU | LYS | HIS | LEU | THR | ASP | ||||
10 | GLY | MET | THR | VAL | ARG | GLU | LEU | CYS | SER | ALA | ||||
11 | ALA | ILE | THR | MET | SER | ASP | ASN | THR | ALA | ALA | ||||
12 | ASN | LEU | LEU | LEU | THR | THR | ILE | GLY | GLY | PRO | ||||
13 | LYS | GLU | LEU | THR | ALA | PHE | LEU | HIS | ASN | MET | ||||
14 | GLY | ASP | HIS | VAL | THR | ARG | LEU | ASP | ARG | TRP | ||||
15 | GLU | PRO | GLU | LEU | ASN | GLU | ALA | ILE | PRO | ASN | ||||
16 | ASP | GLU | ARG | ASP | THR | THR | MET | PRO | ALA | ALA | ||||
17 | MET | ALA | THR | THR | LEU | ARG | LYS | LEU | LEU | THR | ||||
18 | GLY | GLU | LEU | LEU | THR | LEU | ALA | SER | ARG | GLN | ||||
19 | GLN | LEU | ILE | ASP | TRP | MET | GLU | ALA | ASP | LYS | ||||
20 | VAL | ALA | GLY | PRO | LEU | LEU | ARG | SER | ALA | LEU | ||||
21 | PRO | ALA | GLY | TRP | PHE | ILE | ALA | ASP | LYS | SER | ||||
22 | GLY | ALA | GLY | GLU | ARG | GLY | SER | ARG | GLY | ILE | ||||
23 | ILE | ALA | ALA | LEU | GLY | PRO | ASP | GLY | LYS | PRO | ||||
24 | SER | ARG | ILE | VAL | VAL | ILE | TYR | THR | THR | GLY | ||||
25 | SER | GLN | ALA | THR | MET | ASP | GLU | ARG | ASN | ARG | ||||
26 | GLN | ILE | ALA | GLU | ILE | GLY | ALA | SER | LEU | ILE | ||||
27 | LYS | HIS | TRP |
sample_1: TEM-1 beta-lactamase, [U-97.5% 13C; U-97.5% 15N], 1.0 mM; sodium phosphate 25 mM; Imidazole 4.0 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%
sample_2: TEM-1 beta-lactamase, [U-97.5% 15N], 1.0 mM; sodium phosphate 25 mM; Imidazole 4.0 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%
Ex-cond_1: pH: 6.6 na; temperature: 303 K; ionic strength: 0.05 M
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
Chemical shifts were corrected for the TROSY experiment by adding 46.1Hz to the | not available | not available | Ex-cond_1 |
1H shifts and removing 46.1Hz to the 15N shifts. | not available | not available | Ex-cond_1 |
Vnmr v6.1c - Acquisition of NMR experiments
NMRPipe v2.2 - Processing of NMR spectra
NMRView v5.2.2 - Sequential assignment
BMRB | 16392 6024 7236 7237 7238 7239 |
PDB | |
DBJ | BAA00795 BAA03488 BAA12825 BAA14388 BAA19239 |
EMBL | CAA04868 CAA05682 CAA05685 CAA05686 CAA05689 |
GB | AAA24057 AAA25053 AAA32208 AAA53119 AAA53121 |
PIR | S60310 S60312 T51301 |
PRF | 2018199A |
REF | NP_052173 NP_569411 NP_608310 NP_758767 NP_775035 |
SP | P62593 P62594 Q48406 |
TPE | CAJ85677 |
AlphaFold | P62593 P62594 Q48406 P62593 P62594 Q48406 |
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