BMRB Entry 6364
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6364
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Title: 1H, 13C 15N chemical shift assignment of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14 PubMed: 16027363
Deposition date: 2004-10-27 Original release date: 2008-08-28
Authors: Liu, Gaohua; Xiao, Rong; Parish, David; Ma, Lichung; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas
Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination" Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).
Assembly members:
Protein PF0470, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Protein PF0470: MKVYRLYLKDEYLEMVKSGK
KRIEVRVAYPQLKDIKRGDK
IIFNDLIPAEVVEVKKYETF
RQVLREEPIDKIFPDKPSFE
KALKRFHNMYPKWKEYRYGV
LAIKFRVLGRDKE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 815 |
| 13C chemical shifts | 415 |
| 15N chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Protein PF0470 | 1 |
Entities:
Entity 1, Protein PF0470 113 residues - Formula weight is not available
| 1 | MET | LYS | VAL | TYR | ARG | LEU | TYR | LEU | LYS | ASP | ||||
| 2 | GLU | TYR | LEU | GLU | MET | VAL | LYS | SER | GLY | LYS | ||||
| 3 | LYS | ARG | ILE | GLU | VAL | ARG | VAL | ALA | TYR | PRO | ||||
| 4 | GLN | LEU | LYS | ASP | ILE | LYS | ARG | GLY | ASP | LYS | ||||
| 5 | ILE | ILE | PHE | ASN | ASP | LEU | ILE | PRO | ALA | GLU | ||||
| 6 | VAL | VAL | GLU | VAL | LYS | LYS | TYR | GLU | THR | PHE | ||||
| 7 | ARG | GLN | VAL | LEU | ARG | GLU | GLU | PRO | ILE | ASP | ||||
| 8 | LYS | ILE | PHE | PRO | ASP | LYS | PRO | SER | PHE | GLU | ||||
| 9 | LYS | ALA | LEU | LYS | ARG | PHE | HIS | ASN | MET | TYR | ||||
| 10 | PRO | LYS | TRP | LYS | GLU | TYR | ARG | TYR | GLY | VAL | ||||
| 11 | LEU | ALA | ILE | LYS | PHE | ARG | VAL | LEU | GLY | ARG | ||||
| 12 | ASP | LYS | GLU |
Samples:
sample: Protein PF0470, [U-13C; U-15C], 1 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%
sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| GFT (4,3)D HNNCabCa' | not available | not available | not available |
| GFT (4,3)D CabCa(CO)NHN' | not available | not available | not available |
| GFT (4,3)D HabCab(CO)NHN' | not available | not available | not available |
| GFT (4,3) HCCH' | not available | not available | not available |
| Simultaneous Heteronuclear Resolved NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts