BMRB Entry 6371
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6371
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: McCallum, Scott; Yin, JianPing; Fairbrother, Wayne. "Letters to the Editor: 1H, 13C, and 15N resonance assignments of SAP18" J. Biomol. NMR 31, 259-259 (2005).
Assembly members:
Sin3a associated polypeptide p18, polymer, 148 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-28d
Entity Sequences (FASTA):
Sin3a associated polypeptide p18: GSHMRVTQEEIKKEPEKPID
REKTSPLLLRVFTTNNGRHH
RMDEFSRGNVPSSELQIYTW
MDATLKELTSLVKEVYPEAR
KKGTHFNFAIVFTDVKRPGY
RVKEIGSTMSGRKGTDDSMT
LQSQKFQIGDYLDIAITPPN
RAPPPSGR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 992 |
| 13C chemical shifts | 559 |
| 15N chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SAP-18 monomer | 1 |
Entities:
Entity 1, SAP-18 monomer 148 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ARG | VAL | THR | GLN | GLU | GLU | ||||
| 2 | ILE | LYS | LYS | GLU | PRO | GLU | LYS | PRO | ILE | ASP | ||||
| 3 | ARG | GLU | LYS | THR | SER | PRO | LEU | LEU | LEU | ARG | ||||
| 4 | VAL | PHE | THR | THR | ASN | ASN | GLY | ARG | HIS | HIS | ||||
| 5 | ARG | MET | ASP | GLU | PHE | SER | ARG | GLY | ASN | VAL | ||||
| 6 | PRO | SER | SER | GLU | LEU | GLN | ILE | TYR | THR | TRP | ||||
| 7 | MET | ASP | ALA | THR | LEU | LYS | GLU | LEU | THR | SER | ||||
| 8 | LEU | VAL | LYS | GLU | VAL | TYR | PRO | GLU | ALA | ARG | ||||
| 9 | LYS | LYS | GLY | THR | HIS | PHE | ASN | PHE | ALA | ILE | ||||
| 10 | VAL | PHE | THR | ASP | VAL | LYS | ARG | PRO | GLY | TYR | ||||
| 11 | ARG | VAL | LYS | GLU | ILE | GLY | SER | THR | MET | SER | ||||
| 12 | GLY | ARG | LYS | GLY | THR | ASP | ASP | SER | MET | THR | ||||
| 13 | LEU | GLN | SER | GLN | LYS | PHE | GLN | ILE | GLY | ASP | ||||
| 14 | TYR | LEU | ASP | ILE | ALA | ILE | THR | PRO | PRO | ASN | ||||
| 15 | ARG | ALA | PRO | PRO | PRO | SER | GLY | ARG |
Samples:
sample_1: Sin3a associated polypeptide p18, [U-99% 13C; U-99% 15N], 1 mM
Ex-cond_1: pH: 5.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 1H-13C TOCSY | not available | not available | not available |
| 1H-13C COSY | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
Software:
FELIX -
CYANA -
CNX -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB23871 BAB24687 BAB61044 BAC39588 BAE21751 |
| EMBL | CAB09797 CAG33316 CAH90072 |
| GB | AAC51322 AAD41090 AAF21220 AAH06625 AAH30836 |
| REF | NP_001028857 NP_001030544 NP_001129009 NP_001135913 NP_001232121 |
| SP | O00422 O55128 Q3T022 Q5RDT5 |
| TPG | DAA23863 |
| AlphaFold | O00422 O55128 Q3T022 Q5RDT5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks