BMRB Entry 6385
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6385
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for pleckstrin human DEP domain PubMed: 15573383
Deposition date: 2004-11-10 Original release date: 2005-01-14
Authors: Civera, Concepcion; Simon, Bernd; Stier, Gunter; Sattler, Michael; Macias, Maria
Citation: Civera, Concepcion; Simon, Bernd; Stier, Gunter; Sattler, Michael; Macias, Maria. "Structure and dynamics of the human pleckstrin DEP domain: distinct molecular features of a novel DEP domain subfamily." Proteins 58, 354-366 (2005).
Assembly members:
pleckstrin, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
pleckstrin: METIDLGALYLSMKDTEKGI
KELNLEKDKKIFNHCFTGNC
VIDWLVSNQSVRNRQEGLMI
ASSLLNEGYLQPAGDMSKSA
VDGTAENPFLDNPDAFYYFP
DSG
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 1087 |
| 13C chemical shifts | 328 |
| 15N chemical shifts | 112 |
| coupling constants | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DEP monomer | 1 |
Entities:
Entity 1, DEP monomer 103 residues - Formula weight is not available
| 1 | MET | GLU | THR | ILE | ASP | LEU | GLY | ALA | LEU | TYR | ||||
| 2 | LEU | SER | MET | LYS | ASP | THR | GLU | LYS | GLY | ILE | ||||
| 3 | LYS | GLU | LEU | ASN | LEU | GLU | LYS | ASP | LYS | LYS | ||||
| 4 | ILE | PHE | ASN | HIS | CYS | PHE | THR | GLY | ASN | CYS | ||||
| 5 | VAL | ILE | ASP | TRP | LEU | VAL | SER | ASN | GLN | SER | ||||
| 6 | VAL | ARG | ASN | ARG | GLN | GLU | GLY | LEU | MET | ILE | ||||
| 7 | ALA | SER | SER | LEU | LEU | ASN | GLU | GLY | TYR | LEU | ||||
| 8 | GLN | PRO | ALA | GLY | ASP | MET | SER | LYS | SER | ALA | ||||
| 9 | VAL | ASP | GLY | THR | ALA | GLU | ASN | PRO | PHE | LEU | ||||
| 10 | ASP | ASN | PRO | ASP | ALA | PHE | TYR | TYR | PHE | PRO | ||||
| 11 | ASP | SER | GLY |
Samples:
sample_1: pleckstrin, [U-13C; U-15N], 0.5 – 1.5 mM
Ex-cond_1: pH: 6.5; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | sample_1 | not available | Ex-cond_1 |
| HNCA | sample_1 | not available | Ex-cond_1 |
| 1H-15N HSQC | sample_1 | not available | Ex-cond_1 |
| CBCANH | sample_1 | not available | Ex-cond_1 |
| CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
| HNCO | sample_1 | not available | Ex-cond_1 |
| H(CC)(CO)NH | sample_1 | not available | Ex-cond_1 |
| C(CO)NH | sample_1 | not available | Ex-cond_1 |
| HCCH-TOCSY | sample_1 | not available | Ex-cond_1 |
| HBCBCCDHD | sample_1 | not available | Ex-cond_1 |
| HBCBCCDCEHE | sample_1 | not available | Ex-cond_1 |
| 2D TOCSY | sample_1 | not available | Ex-cond_1 |
| 2DNOESY | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- BRUKER DRX 500 MHz
- BRUKER DRX 600 MHz