BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6406

Title: Backbone and side chain chemical shift assignments of a TRAV14-3 mouse T cell receptor domain

Deposition date: 2004-11-30 Original release date: 2005-01-14

Authors: Hu, Jin-Shan

Citation: Hu, Jin-Shan; Plaksin, Daniel; Margulies, David. "Letter to the Editor: Backbone and side chain resonance assignments of a TRAV14-3 mouse T cell receptor domain"  J. Biomol. NMR 31, 271-272 (2005).

Assembly members:
TCR Va2.6 domain, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TCR Va2.6 domain: MQQVRQSPQSLTVWEGETAI LNCSYENSAFDYFPWYQQFP GEGPALLISILSVSNKKEDG RFTIFFNKREKKLSLHIADS QPGDSATYFCAASASFGDNS KLIWGLGTSLVVNP

Data sets:
Data typeCount
1H chemical shifts760
13C chemical shifts489
15N chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Va2.6 monomer1

Entities:

Entity 1, Va2.6 monomer 114 residues - Formula weight is not available

1   METGLNGLNVALARGGLNSERPROGLNSER
2   LEUTHRVALTRPGLUGLYGLUTHRALAILE
3   LEUASNCYSSERTYRGLUASNSERALAPHE
4   ASPTYRPHEPROTRPTYRGLNGLNPHEPRO
5   GLYGLUGLYPROALALEULEUILESERILE
6   LEUSERVALSERASNLYSLYSGLUASPGLY
7   ARGPHETHRILEPHEPHEASNLYSARGGLU
8   LYSLYSLEUSERLEUHISILEALAASPSER
9   GLNPROGLYASPSERALATHRTYRPHECYS
10   ALAALASERALASERPHEGLYASPASNSER
11   LYSLEUILETRPGLYLEUGLYTHRSERLEU
12   VALVALASNPRO

Samples:

sample_1: TCR Va2.6 domain, [U-95% 15N], 0.94 mM

sample_2: TCR Va2.6 domain, [U-95% 13C; U-95% 15N], 0.91 mM

Ex-cond_1: pH: 7.0; temperature: 300 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCOCOnot availablenot availableEx-cond_1
HNCG aromnot availablenot availableEx-cond_1
HN(CO)CG aromnot availablenot availableEx-cond_1
HNCGnot availablenot availableEx-cond_1
HN(CO)Cnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HBHA(CO)NHnot availablenot availableEx-cond_1
DIPSI-H(CCO)NHnot availablenot availableEx-cond_1
DIPSI-C(CCO)NHnot availablenot availableEx-cond_1
HAHBnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCCH-COSYnot availablenot availableEx-cond_1
1H-13C HSQCnot availablenot availableEx-cond_1
15N-HOHAHA-HSQCnot availablenot availableEx-cond_1
15N-NOESY-HSQCnot availablenot availableEx-cond_1
13C-15N NOESYnot availablenot availableEx-cond_1
13C-13C NOESYnot availablenot availableEx-cond_1

Software:

NMRPIPE - data processing, analysis

PIPP - data processing, analysis

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
GB AAA57548 AAB16801 AAI47847 AAI47853 AAL08199

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts