BMRB Entry 6409
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6409
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Title: Backbone 1H, 13C, and 15N and 13CB Chemical Shift Assignments for Four N-terminal C2H2 Zinc Fingers (F1-4) of MTF-1 PubMed: 16055450
Deposition date: 2004-12-03 Original release date: 2005-11-14
Authors: Laity, John; Potter, Belinda; Knudsen, Nathan
Citation: Potter, Belinda; Feng, Linda; Parasuram, Priya; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "The six zinc fingers of metal-responsive element binding transcription factor-1 form stable and quasi-ordered structures with relatively small differences in zinc affinities." J. Biol. Chem. 280, 28529-28540 (2005).
Assembly members:
metal-responsive element-binding transcription factor-1, polymer, 117 residues, 13652.5 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
metal-responsive element-binding transcription factor-1: VKRYQCTFEGCPRTYSTAGN
LRTHQKTHRGEYTFVCNQEG
CGKAFLTSYSLRIHVRVHTK
EKPFECDVQGCEKAFNTLYR
LKAHQRLHTGKTFNCESQGC
SKYFTTLSDLRKHIRTH
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 108 |
13C chemical shifts | 321 |
15N chemical shifts | 108 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | MTF-1 subunit 1 | 1 |
2 | Zn (II) A | 2 |
3 | Zn (II) B | 2 |
4 | Zn (II) C | 2 |
5 | Zn (II) D | 2 |
Entities:
Entity 1, MTF-1 subunit 1 117 residues - 13652.5 Da.
1 | VAL | LYS | ARG | TYR | GLN | CYS | THR | PHE | GLU | GLY | ||||
2 | CYS | PRO | ARG | THR | TYR | SER | THR | ALA | GLY | ASN | ||||
3 | LEU | ARG | THR | HIS | GLN | LYS | THR | HIS | ARG | GLY | ||||
4 | GLU | TYR | THR | PHE | VAL | CYS | ASN | GLN | GLU | GLY | ||||
5 | CYS | GLY | LYS | ALA | PHE | LEU | THR | SER | TYR | SER | ||||
6 | LEU | ARG | ILE | HIS | VAL | ARG | VAL | HIS | THR | LYS | ||||
7 | GLU | LYS | PRO | PHE | GLU | CYS | ASP | VAL | GLN | GLY | ||||
8 | CYS | GLU | LYS | ALA | PHE | ASN | THR | LEU | TYR | ARG | ||||
9 | LEU | LYS | ALA | HIS | GLN | ARG | LEU | HIS | THR | GLY | ||||
10 | LYS | THR | PHE | ASN | CYS | GLU | SER | GLN | GLY | CYS | ||||
11 | SER | LYS | TYR | PHE | THR | THR | LEU | SER | ASP | LEU | ||||
12 | ARG | LYS | HIS | ILE | ARG | THR | HIS |
Entity 2, Zn (II) A - Zn - 65.409 Da.
1 | ZN |
Samples:
all_samples: metal-responsive element-binding transcription factor-1, [U-98% 13C; U-98% 15N], 0.7 1.0 mM; B-mercaptoethanol 5 mM; MES 20 mM; H2O 95%; D2O 5%
cond_1: pH: 6.9; temperature: 293 K; ionic strength: 0.01 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N HSQC | all_samples | not available | cond_1 |
HNCACB | all_samples | not available | cond_1 |
CBCA(CO)NH | all_samples | not available | cond_1 |
HNCO | all_samples | not available | cond_1 |
HNCA | all_samples | not available | cond_1 |
Software:
NMRView v5.0.4 - Data analysis
NMRPipe vNMRPipe - Raw spectral data processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
SWISS-PROT | Q07243 Q14872 |
REF | XP_001503611.1 XP_513329.2 NP_032662.2 NP_001030252.2 NP_001102147.1 |
GenBank | EDL80405.1 AAH17679.1 EDL30350.1 AAP31813.1 EDL30349.1 |
EMBL | CAH70087.1 CAA55363.1 CAA50470.1 CAB71344.1 |
DBJ | BAE00336.1 BAC26879.1 BAA22262.1 |
BMRB | 6445 6275 6276 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts