BMRB Entry 6431
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6431
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Title: 1H, 13C and 15N assignments for the II-III loop region of the skeletal dyhydropyridine receptor PubMed: 16041487
Deposition date: 2004-12-13 Original release date: 2005-08-16
Authors: Cui, Yanfang; Karunasekara, Yamuna; Harvey, Peta; Board, Philip; Dulhunty, Angela; Casarotto, Marco
Citation: Cui, Yanfang; Karunasekara, Yamuna; Harvey, Peta; Board, Philip; Dulhunty, Angela; Casarotto, Marco. "Letter to the Editor: 1H, 13C and 15N assignments for the II-III loop region of the skeletal dyhydropyridine receptor" J. Biomol. NMR 32, 89-90 (2005).
Assembly members:
II-III loop region of the skeletal dyhydropyridine receptor, polymer, 126 residues, 14134 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
II-III loop region of the skeletal dyhydropyridine receptor: EAESLTSAQKAKAEERKRRK
MSRGLPDKTEEEKSVMAKKL
EQKPKGEGIPTTAKLKVDEF
ESNVNEVKDPYPSADFPGDD
EEDEPEIPVSPRPRPLAELQ
LKEKAVPIPEASSFFIFSPT
NKVRVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 510 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 813 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | II-III loop | 1 |
Entities:
Entity 1, II-III loop 126 residues - 14134 Da.
| 1 | GLU | ALA | GLU | SER | LEU | THR | SER | ALA | GLN | LYS | ||||
| 2 | ALA | LYS | ALA | GLU | GLU | ARG | LYS | ARG | ARG | LYS | ||||
| 3 | MET | SER | ARG | GLY | LEU | PRO | ASP | LYS | THR | GLU | ||||
| 4 | GLU | GLU | LYS | SER | VAL | MET | ALA | LYS | LYS | LEU | ||||
| 5 | GLU | GLN | LYS | PRO | LYS | GLY | GLU | GLY | ILE | PRO | ||||
| 6 | THR | THR | ALA | LYS | LEU | LYS | VAL | ASP | GLU | PHE | ||||
| 7 | GLU | SER | ASN | VAL | ASN | GLU | VAL | LYS | ASP | PRO | ||||
| 8 | TYR | PRO | SER | ALA | ASP | PHE | PRO | GLY | ASP | ASP | ||||
| 9 | GLU | GLU | ASP | GLU | PRO | GLU | ILE | PRO | VAL | SER | ||||
| 10 | PRO | ARG | PRO | ARG | PRO | LEU | ALA | GLU | LEU | GLN | ||||
| 11 | LEU | LYS | GLU | LYS | ALA | VAL | PRO | ILE | PRO | GLU | ||||
| 12 | ALA | SER | SER | PHE | PHE | ILE | PHE | SER | PRO | THR | ||||
| 13 | ASN | LYS | VAL | ARG | VAL | LEU |
Samples:
sample_1: II-III loop region of the skeletal dyhydropyridine receptor, [U-95% 13C; U-90% 15N], 1 mM
Cond-1: pH: 6.5; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | sample_1 | not available | Cond-1 |
| 1H-15N TOCSY | sample_1 | not available | Cond-1 |
| 1H-13C NOESY | sample_1 | not available | Cond-1 |
| HNCACB | sample_1 | not available | Cond-1 |
| CACB(CO)NH | sample_1 | not available | Cond-1 |
| HNCO | sample_1 | not available | Cond-1 |
| HCCH-TOCSY | sample_1 | not available | Cond-1 |
| HCAN | sample_1 | not available | Cond-1 |
| HCACB(CO)N | sample_1 | not available | Cond-1 |
Software:
FELIX -
SPARKY -
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| EMBL | CAA29355 |
| GB | AAA31159 |
| PRF | 1310328A |
| REF | NP_001095190 XP_004578840 |
| SP | P07293 |
| AlphaFold | P07293 |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
or all simulated shifts