BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6441

Title: Chemical shift assignments for human Orexin-A   PubMed: 16429482

Deposition date: 2004-12-22 Original release date: 2006-04-17

Authors: Ikegami, Takahisa; Takai, Tomoyo

Citation: Takai, Tomoyo; Takaya, Takao; Nakano, Mutsuko; Akutsu, Hideo; Nakagawa, Atsushi; Aimoto, Saburo; Nagai, Katsuya; Ikegami, Takahisa. "Orexin-A is composed of a highly conserved C-terminal and a specific, hydrophilic N-terminal region, revealing the structural basis of specific recognition by the orexin-1 receptor."  J. Pept. Sci. 12, 443-454 (2006).

Assembly members:
human Orexin-A, polymer, 33 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
human Orexin-A: XPLPDCCRQKTCSCRLYELL HGAGNHAAGILTX

Data sets:
Data typeCount
13C chemical shifts54
15N chemical shifts31
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Orexin-A1

Entities:

Entity 1, human Orexin-A 33 residues - Formula weight is not available

1   PCAPROLEUPROASPCYSCYSARGGLNLYS
2   THRCYSSERCYSARGLEUTYRGLULEULEU
3   HISGLYALAGLYASNHISALAALAGLYILE
4   LEUTHRNLW

Samples:

sample_1: human Orexin-A 0.76 mM

conditions_1: ionic strength: 18 mM; pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1not availableconditions_1
2D NOESYsample_1not availableconditions_1
DQF-COSYsample_1not availableconditions_1
15N-1H-HSQCsample_1not availableconditions_1
13C-1H-HSQCsample_1not availableconditions_1

Software:

Sparky - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 5994
PDB
DBJ BAB91446.1
EMBL CAM19469.1 CAM19468.1
GenBank AAL17613.1 AAC26827.1 ABJ15704.1 ABO15568.1 EDM06064.1
REF XP_001166578.1 NP_001515.1 NP_999321.1 XP_001108991.1 XP_609741.2
SWISS-PROT O55232 O55241 Q9GLF6 O43612 O77668

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts