BMRB Entry 6516
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6516
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Title: NMR assignment of the holo-acyl carrier protein (PfACP) from malaria parasite Plasmodium falciparum PubMed: 16132833
Deposition date: 2005-02-19 Original release date: 2005-09-01
Authors: Sharma, Alok; Sharma, Shailendra; Surolia, Namita; Sarma, Siddhartha
Citation: Sharma, Alok; Sharma, Shailendra; Surolia, Namita; Sarma, Siddhartha. "NMR Assignment of the Holo-ACP from Malaria Parasite Plasmodium Falciparum" J. Biomol. NMR 32, 260-260 (2005).
Assembly members:
holo-acyl carrier protein from Plasmodium falciparum, polymer, 79 residues, 9006.2 Da.
PNS, non-polymer, 358.348 Da.
Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Plasmodium falciparum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
holo-acyl carrier protein from Plasmodium falciparum: LKSTFDDIKKIISKQLSVEE
DKIQMNSNFTKDLGADSLDL
VELIMALEEKFNVTISDQDA
LKINTVQDAIDYIEKNNKQ
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 352 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 617 |
| coupling constants | 79 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Polypeptide from Plasmodium falciparum, major conformer | 1 |
| 2 | Polypeptide from Plasmodium falciparum, minor conformer | 1 |
| 3 | 4'-phosphopantetheine prosthetic group, major conformer | 2 |
| 4 | 4'-phosphopantetheine prosthetic group, minor conformer | 2 |
Entities:
Entity 1, Polypeptide from Plasmodium falciparum, major conformer 79 residues - 9006.2 Da.
| 1 | LEU | LYS | SER | THR | PHE | ASP | ASP | ILE | LYS | LYS | ||||
| 2 | ILE | ILE | SER | LYS | GLN | LEU | SER | VAL | GLU | GLU | ||||
| 3 | ASP | LYS | ILE | GLN | MET | ASN | SER | ASN | PHE | THR | ||||
| 4 | LYS | ASP | LEU | GLY | ALA | ASP | SER | LEU | ASP | LEU | ||||
| 5 | VAL | GLU | LEU | ILE | MET | ALA | LEU | GLU | GLU | LYS | ||||
| 6 | PHE | ASN | VAL | THR | ILE | SER | ASP | GLN | ASP | ALA | ||||
| 7 | LEU | LYS | ILE | ASN | THR | VAL | GLN | ASP | ALA | ILE | ||||
| 8 | ASP | TYR | ILE | GLU | LYS | ASN | ASN | LYS | GLN |
Entity 2, 4'-phosphopantetheine prosthetic group, major conformer - C11 H23 N2 O7 P S - 358.348 Da.
| 1 | PNS |
Samples:
sample_1: holo-acyl carrier protein from Plasmodium falciparum, [U-98% 13C; U-98% 15N], 1.2 ± 0.2 mM; 4'-phosphopantetheine prosthetic group, [U-98% 13C; U-98% 15N], 1.2 ± 0.2 mM
conditions_1: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 1H-15N TOCSY-HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| H(CCO)NH | sample_1 | not available | conditions_1 |
| C(CCO)HN | sample_1 | not available | conditions_1 |
| HCCH_TOCSY | sample_1 | not available | conditions_1 |
| 1H-13C HSQC | sample_1 | not available | conditions_1 |
| 1H-15N 3D NOESY | sample_1 | not available | conditions_1 |
| 1H-13C 3D NOESY | sample_1 | not available | conditions_1 |
Software:
NMRPipe -
ANSIG -
NMR spectrometers:
- Bruker Avance 700 MHz MHz
Related Database Links:
| BMRB | 16506 16529 16530 16531 16532 16533 16661 |
| PDB | |
| EMBL | CDO62314 |
| GB | AAC63959 AAC71866 ETW20884 ETW27965 ETW39040 |
| REF | XP_001349595 |
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