BMRB Entry 6560

Name: The NMR solution structure of a redesigned apocytochrome b562:Rd-apocyt b562
Published: 2005-08-30

Click here to enlarge.

PDB ID: 1yyj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6560
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

The NMR solution structure of a redesigned apocytochrome b562:Rd-apocyt b562

Deposition date:

2005-03-18

Original release date:

2005-08-30

Authors:

Feng, H.; Takei, J.; Lipsitz, R.; Tjandra, N.; Bai, Y.

Citation:

Citation: Feng, H.; Takei, J.; Lipsitz, R.; Tjandra, N.; Bai, Y.. "Specific non-native hydrophobic interactions in a hidden folding intermediate: implications for protein folding" Biochemistry 42, 12461-12465 (2003).
PubMed: 14580191

Assembly members:

Assembly members:
redesigned apocytochrome B562, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homon sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
redesigned apocytochrome B562: ADLEDNWETLNDNLKVIEKA DNAAQVKDALTKMRAAALDA QKATPPKLEDKSPDSPEMKD FRHGFDILVGQIDDALKLAN EGKVKEAQAAAEQLKTTIRA YNQKYG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	331
15N chemical shifts	110
1H chemical shifts	713
coupling constants	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	redesigned apocytochrome B562	1

Entities:

Entity 1, redesigned apocytochrome B562 106 residues - Formula weight is not available

1

ALA

ASP

LEU

GLU

ASP

ASN

TRP

GLU

THR

LEU

2

ASN

ASP

ASN

LEU

LYS

VAL

ILE

GLU

LYS

ALA

3

ASP

ASN

ALA

GLN

VAL

LYS

ASP

ALA

LEU

4

THR

LYS

MET

ARG

ALA

LEU

ASP

ALA

5

GLN

LYS

ALA

THR

PRO

LYS

LEU

GLU

ASP

6

LYS

SER

PRO

ASP

SER

PRO

GLU

MET

LYS

ASP

7

PHE

ARG

HIS

GLY

PHE

ASP

ILE

LEU

VAL

GLY

8

GLN

ILE

ASP

ALA

LEU

LYS

LEU

ALA

ASN

9

GLU

GLY

LYS

VAL

LYS

GLU

ALA

GLN

ALA

10

ALA

GLU

GLN

LEU

LYS

THR

ILE

ARG

ALA

11

TYR

ASN

GLN

LYS

TYR

GLY

Samples:

sample_1: redesigned apocytochrome B562, [U-15N; U-13C], 1.5 mM; Sodium acetate 50 mM; H2O 92.5%; D2O 7.5%

sample_2: redesigned apocytochrome B562, [U-13C], 1.5 mM; Sodium acetate 50 mM; H2O 92.5%; D2O 7.5%

sample_cond_1: pH: 4.84; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D HSQC	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available
3D HNCACB	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HBHA(CO)NH	not available	not available	not available

Software:

X-PLOR vXPLOR-NIH-2.9 - refinement, structure solution

NMRPipe - processing

NMR spectrometers:

Bruker DRX 750 MHz

PDB	1M6T 1YYJ 1YYX 4EA3 4EIY 4IAQ 4IAR 4IB4 4JKV 4L6R 4N4W 4N6H 4NC3 4NTJ 4O9R 4OR2 4PXZ 4PY0 4QIM 4QIN 4RWA 4RWD
EMBL	CDX09681
GB	AAN45672 AIL38642 AIL43578 EFS10896 EGJ79391
REF	NP_709965 WP_005086530