BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6574

Title: MrR5

Deposition date: 2005-03-31 Original release date: 2005-12-09

Authors: Rossi, P.; Aramini, J.; Xiao, R.; Ho, C.; Ma, L.; Acton, T.; Montelione, G.

Citation: Rossi, P.; Aramini, J.; Xiao, R.; Ho, C.; Ma, L.; Acton, T.; Montelione, G.. "."  .

Assembly members:
MrR5, polymer, 70 residues, Formula weight is not available

Natural source:   Common Name: Methanococcus maripaludis   Taxonomy ID: 39152   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus maripaludis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MrR5: MAFGKPAMKNVPVEAGKEYE VTIEDMGKGGDGIARIDGFV VFVPNAEKGSVINVKVTAVK EKFAFAERVL

Data sets:
Data typeCount
1H chemical shifts482
13C chemical shifts306
15N chemical shifts68
coupling constants57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MrR51

Entities:

Entity 1, MrR5 70 residues - Formula weight is not available

1   METALAPHEGLYLYSPROALAMETLYSASN
2   VALPROVALGLUALAGLYLYSGLUTYRGLU
3   VALTHRILEGLUASPMETGLYLYSGLYGLY
4   ASPGLYILEALAARGILEASPGLYPHEVAL
5   VALPHEVALPROASNALAGLULYSGLYSER
6   VALILEASNVALLYSVALTHRALAVALLYS
7   GLULYSPHEALAPHEALAGLUARGVALLEU

Samples:

sample_1: MrR5, [U-15N; U-5% 13C], 1.1 mM; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_2: MrR5, [U-15N; U-13C], 1.2 mM; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: pH: 4.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HcCH-COSYnot availablenot availablenot available
Hi-Res CH-HSQC(st. Me assign)not availablenot availablenot available
HNHAnot availablenot availablenot available
HetNOEnot availablenot availablenot available
TR backbonenot availablenot availablenot available
H,C_TOCSYsnot availablenot availablenot available
NH-HSQCnot availablenot availablenot available

Software:

VNMR v6.1 - collection

XWINNMR v3.5 - collection

NMRPipe v2.1 - processing

Sparky v3.91 - data analysis

AutoAssign v1.15.1 - data analysis

AutoStructure v2.1.0 - structure solution, refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAP60196 BAP62190
EMBL CAF29632
GB ABO35898 ABR66141 ABX01687 AEK18968
REF NP_987196 WP_011170020 WP_011869345 YP_001098112 YP_001330292

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts