BMRB Entry 6574
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6574
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rossi, P.; Aramini, J.; Xiao, R.; Ho, C.; Ma, L.; Acton, T.; Montelione, G.. "." .
Assembly members:
MrR5, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: Methanococcus maripaludis Taxonomy ID: 39152 Superkingdom: Archaea Kingdom: not available Genus/species: Methanococcus maripaludis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MrR5: MAFGKPAMKNVPVEAGKEYE
VTIEDMGKGGDGIARIDGFV
VFVPNAEKGSVINVKVTAVK
EKFAFAERVL
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 482 |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 68 |
| coupling constants | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MrR5 | 1 |
Entities:
Entity 1, MrR5 70 residues - Formula weight is not available
| 1 | MET | ALA | PHE | GLY | LYS | PRO | ALA | MET | LYS | ASN | |
| 2 | VAL | PRO | VAL | GLU | ALA | GLY | LYS | GLU | TYR | GLU | |
| 3 | VAL | THR | ILE | GLU | ASP | MET | GLY | LYS | GLY | GLY | |
| 4 | ASP | GLY | ILE | ALA | ARG | ILE | ASP | GLY | PHE | VAL | |
| 5 | VAL | PHE | VAL | PRO | ASN | ALA | GLU | LYS | GLY | SER | |
| 6 | VAL | ILE | ASN | VAL | LYS | VAL | THR | ALA | VAL | LYS | |
| 7 | GLU | LYS | PHE | ALA | PHE | ALA | GLU | ARG | VAL | LEU |
Samples:
sample_1: MrR5, [U-15N; U-5% 13C], 1.1 mM; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_2: MrR5, [U-15N; U-13C], 1.2 mM; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_cond_1: pH: 4.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HcCH-COSY | not available | not available | not available |
| Hi-Res CH-HSQC(st. Me assign) | not available | not available | not available |
| HNHA | not available | not available | not available |
| HetNOE | not available | not available | not available |
| TR backbone | not available | not available | not available |
| H,C_TOCSYs | not available | not available | not available |
| NH-HSQC | not available | not available | not available |
Software:
VNMR v6.1 - collection
XWINNMR v3.5 - collection
NMRPipe v2.1 - processing
Sparky v3.91 - data analysis
AutoAssign v1.15.1 - data analysis
AutoStructure v2.1.0 - structure solution, refinement
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAP60196 BAP62190 |
| EMBL | CAF29632 |
| GB | ABO35898 ABR66141 ABX01687 AEK18968 |
| REF | NP_987196 WP_011170020 WP_011869345 YP_001098112 YP_001330292 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks