BMRB Entry 6575
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6575
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Title: Backbone and side-chain chemical shift assignments for Human Nck2 SH2 domain PubMed: 15764601
Deposition date: 2005-04-01 Original release date: 2005-06-02
Authors: Ran, Xiaoyuan; Song, Jianxing
Citation: Ran, Xiaoyuan; Song, Jianxing. "Structural Insight into the Binding Diversity between the Tyr-phosphorylated Human EphrinBs and Nck2 SH2 Domain." J. Biol. Chem. 280, 19205-19212 (2005).
Assembly members:
Nck2 SH2 domain, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo human
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Nck2 SH2 domain: REWYYGNVTRHQAECALNER
GVEGDFLIRDSESSPSDFSV
SLKASGKNKHFKVQLVDNVY
CIGQRRFHTMDELVEHYKKA
PIFTSEHGEKLYLVRALQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 370 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 433 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nck2 SH2 domain | 1 |
Entities:
Entity 1, Nck2 SH2 domain 98 residues - Formula weight is not available
| 1 | ARG | GLU | TRP | TYR | TYR | GLY | ASN | VAL | THR | ARG | ||||
| 2 | HIS | GLN | ALA | GLU | CYS | ALA | LEU | ASN | GLU | ARG | ||||
| 3 | GLY | VAL | GLU | GLY | ASP | PHE | LEU | ILE | ARG | ASP | ||||
| 4 | SER | GLU | SER | SER | PRO | SER | ASP | PHE | SER | VAL | ||||
| 5 | SER | LEU | LYS | ALA | SER | GLY | LYS | ASN | LYS | HIS | ||||
| 6 | PHE | LYS | VAL | GLN | LEU | VAL | ASP | ASN | VAL | TYR | ||||
| 7 | CYS | ILE | GLY | GLN | ARG | ARG | PHE | HIS | THR | MET | ||||
| 8 | ASP | GLU | LEU | VAL | GLU | HIS | TYR | LYS | LYS | ALA | ||||
| 9 | PRO | ILE | PHE | THR | SER | GLU | HIS | GLY | GLU | LYS | ||||
| 10 | LEU | TYR | LEU | VAL | ARG | ALA | LEU | GLN |
Samples:
sample_1: Nck2 SH2 domain mM
conditions_1: pH: 6.8; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
Related Database Links:
| PDB | |
| DBJ | BAI47129 |
| EMBL | CAG31895 |
| GB | AAC04831 AAC06353 AAC80284 AAH00103 AAH07195 |
| PIR | B46243 |
| REF | NP_001004720 NP_001025985 NP_001092506 NP_001101686 NP_001131103 |
| SP | O43639 O55033 |
| TPG | DAA24593 |
| AlphaFold | O55033 O43639 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts