BMRB Entry 6576
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6576
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Title: Backbone and sidechain 1H assignments of Crotamine
Deposition date: 2005-04-01 Original release date: 2005-10-28
Authors: Fadel, Valmir; Bettendorff, Pascal; Herrmann, Torsten; de Azevedo, Walter; Oliveira, Eduardo; Yamane, Tetsuo; Wuthrich, Kurt
Citation: Fadel, Valmir; Bettendorff, Pascal; Herrmann, Torsten; de Azevedo, Walter; Oliveira, Eduardo; Yamane, Tetsuo; Wuthrich, Kurt. "Automated NMR structure determination and disulfide bond identification of the myotoxin crotamine from Crotalus durissus terrificus" Toxicon ., .-. (2005).
Assembly members:
Crotamine, polymer, 42 residues, Formula weight is not available
Natural source: Common Name: tropical rattlesnake Taxonomy ID: 8732 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Crotalus durissus terrificus
Experimental source: Production method: Purified from the natural source
Entity Sequences (FASTA):
Crotamine: YKQCHKKGGHCFPKEKICLP
PSSDFGKMDCRWRWKCCKKG
SG
- assigned_chemical_shifts
- coupling_constants
Data type | Count |
1H chemical shifts | 510 |
coupling constants | 22 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Crotamine | 1 |
Entities:
Entity 1, Crotamine 42 residues - Formula weight is not available
1 | TYR | LYS | GLN | CYS | HIS | LYS | LYS | GLY | GLY | HIS | ||||
2 | CYS | PHE | PRO | LYS | GLU | LYS | ILE | CYS | LEU | PRO | ||||
3 | PRO | SER | SER | ASP | PHE | GLY | LYS | MET | ASP | CYS | ||||
4 | ARG | TRP | ARG | TRP | LYS | CYS | CYS | LYS | LYS | GLY | ||||
5 | SER | GLY |
Samples:
D2O_sample: Crotamine, no, 1.8 ± 0.2 mM; D2O 100%
sample: Crotamine, no, 1.8 ± 0.2 mM; D2O 5%; H2O 95%
higher_temperature: pH: 5.8; temperature: 313.4 K
room_temperature: pH: 5.8; temperature: 293.4 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
[1H,_1H]-DQF-COSY | not available | not available | not available |
[1H,_1H]-TOCSY | not available | not available | not available |
[1H,_1H]-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
- Bruker Avance 900 MHz