BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6589

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chromo Domain of cpSRP43   PubMed: 16183644

Deposition date: 2005-04-12 Original release date: 2007-02-05

Authors: Vaithiyalingam, Sivaraja; Thallapuranam, Suresh Kumar; Philominathan, Leena; Henry, Ralph; Yu, Chin

Citation: Sivaraja, Vaithiyalingam; Suresh Kumar, Thallapuranam; Leena, Philominathan; Chang, Anne; Vidya, Chitturi; Goforth, Robyn; Rajalingam, Dakshinamurthy; Arvind, Kannan; Ye, Jian-Liang; Chou, Jonathan; Henry, Ralph; Yu, Chin. "Three-dimensional solution structures of the chromodomains of cpSRP43"  J. Biol. Chem. 280, 41465-41471 (2005).

Assembly members:
Chromo 1, polymer, 47 residues, Formula weight is not available

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eubacteria   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Chromo 1: GSGEVNKIIGSRTAGEGAME YLIEWKDGHSPSWVPSSYIA ADVVSEY

Data sets:
Data typeCount
13C chemical shifts107
15N chemical shifts44
1H chemical shifts237

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Chromo domain 1 of cpSRP431

Entities:

Entity 1, Chromo domain 1 of cpSRP43 47 residues - Formula weight is not available

1   GLYSERGLYGLUVALASNLYSILEILEGLY
2   SERARGTHRALAGLYGLUGLYALAMETGLU
3   TYRLEUILEGLUTRPLYSASPGLYHISSER
4   PROSERTRPVALPROSERSERTYRILEALA
5   ALAASPVALVALSERGLUTYR

Samples:

CD1_-_Chromo_1_domain: CD1 - Chromo domain 1, [U-15N; U-13C], 0.75 ± 0.01 mM; phosphate buffer 100 mM; NaCl 100 mM

CD1_sample: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC (example)CD1_-_Chromo_1_domainnot availableCD1_sample
1H 2D NOESYCD1_-_Chromo_1_domainnot availableCD1_sample
1H 2D TOCSYCD1_-_Chromo_1_domainnot availableCD1_sample
HNCACD1_-_Chromo_1_domainnot availableCD1_sample
HNCOCACD1_-_Chromo_1_domainnot availableCD1_sample
CBCACONHCD1_-_Chromo_1_domainnot availableCD1_sample
3D 15N NOESY-HSQCCD1_-_Chromo_1_domainnot availableCD1_sample

Software:

XWIN NMR v3.5, Bruker -

NMR spectrometers:

  • Bruker DMX 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts