BMRB Entry 6648

Name: Solution Structure of a Zap1 Zinc-Responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae
Published: 2006-04-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6648

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution Structure of a Zap1 Zinc-Responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae

Deposition date: 2005-05-25 Original release date: 2006-04-27

Authors: Wang, Zhonghua; Feng, Linda; Venkataraman, Krishna; Matskevich, Viktor; Parasuram, Priya; Laity, John

Citation: Wang, Zhonghua; Feng, Linda; Matskevich, Viktor; Venkataraman, Krishna; Parasuram, Priya; Laity, John. "Solution Structure of a Zap1 Zinc-responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae" J. Mol. Biol. 357, 1167-1183 (2006).

Assembly members:
Zap1-2, polymer, 64 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: recombinant technology Vector: p21a-zzf1-2

Entity Sequences (FASTA):
Zap1-2: DLKCKWKECPESASSLFDLQ RHLLKDHVSQDFKHPMEPLA CNWEDCDFLGDDTASIVNHI NAQH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	64
1H chemical shifts	425

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	64 amino acid polypeptide	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, 64 amino acid polypeptide 64 residues - Formula weight is not available

1

ASP

LEU

LYS

CYS

LYS

TRP

LYS

GLU

CYS

PRO

2

GLU

SER

ALA

SER

LEU

PHE

ASP

LEU

GLN

3

ARG

HIS

LEU

LYS

ASP

HIS

VAL

SER

GLN

4

ASP

PHE

LYS

HIS

PRO

MET

GLU

PRO

LEU

ALA

5

CYS

ASN

TRP

GLU

ASP

CYS

ASP

PHE

LEU

GLY

6

ASP

THR

ALA

SER

ILE

VAL

ASN

HIS

ILE

7

ASN

ALA

GLN

HIS

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Zap1-2, [U-13C; U-15N], 0.7 mM; ZN 1.5 mM; MES 20 mM; BME 5 mM; DSS 0.2 mM; NaN2 1 mM

conditions_1: pH: 6.9; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N_HSQC	sample_1	not available	conditions_1
3D HNCACB	sample_1	not available	conditions_1
3D CBCA(CO)NH	sample_1	not available	conditions_1
3D HNCO	sample_1	not available	conditions_1
3D H(CA)CO(CA)NH	sample_1	not available	conditions_1
3D HNCA	sample_1	not available	conditions_1
3D HCCH-TOCSY	sample_1	not available	conditions_1
3D HCCH-COSY	sample_1	not available	conditions_1
3D C13NOESY-HSQC-sw_center 45ppm	sample_1	not available	conditions_1
3D N15NOESY-HSQC	sample_1	not available	conditions_1
3D C13NOESY-HSQC-sw_center 125ppm	sample_1	not available	conditions_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 - Raw spectral data processing

NMR spectrometers:

Varian Inova 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts