BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6679

Title: G311   PubMed: 16245921

Deposition date: 2005-06-08 Original release date: 2005-11-03

Authors: He, Yanan; Yeh, Cheon; Alexander, Patrick; Bryan, Philip; Orban, John

Citation: He, Yanan; Yeh, D.; Alexander, Patrick; Bryan, Philip; Orban, John. "Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds"  Biochemistry 44, 14055-14061 (2005).

Assembly members:
G311, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
G311: MYYLVVNKGQNAFYETLTKA VDAETARNAFIQSLKDDGVQ GVWTYDDATKTFTVQA

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts60
1H chemical shifts355

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G3111

Entities:

Entity 1, G311 56 residues - Formula weight is not available

1   METTYRTYRLEUVALVALASNLYSGLYGLN
2   ASNALAPHETYRGLUTHRLEUTHRLYSALA
3   VALASPALAGLUTHRALAARGASNALAPHE
4   ILEGLNSERLEULYSASPASPGLYVALGLN
5   GLYVALTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALGLNALA

Samples:

sample_1: G311, [U-13C; U-15N], 0.2 – 0.4 mM; postassium phosphate buffer 100 mM; D2O 10%

sample_2: G311, [U-15N], 0.2 – 0.4 mM; postassium phosphate buffer 100 mM; D2O 10%

conditions_1: pH: 7; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availableconditions_1
3D HNCOnot availablenot availableconditions_1
3D CBCACONHnot availablenot availableconditions_1
3D HBHA(CBCACO)NHnot availablenot availableconditions_1
3D(H)C(CO)NH-TOCSYnot availablenot availableconditions_1
3d h(CCO)NH-TOCSYnot availablenot availableconditions_1
2D TOCSYnot availablenot availableconditions_1
2D CBHDnot availablenot availableconditions_1
2D CBHEnot availablenot availableconditions_1
2D TOCSYnot availablenot availableconditions_1

Software:

xwinnmr v2.5, Bruker -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts