BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6680

Title: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for A219.   PubMed: 16245921

Deposition date: 2005-06-08 Original release date: 2005-11-03

Authors: He, Yanan; Yeh, Cheon; Alexander, Patrick; Bryan, Philip; Orban, John

Citation: He, Yanan; Yeh, D.; Alexander, Patrick; Bryan, Philip; Orban, John. "Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds"  Biochemistry 44, 14055-14061 (2005).

Assembly members:
A219, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
A219: MYYLVVNKQQNAFYEVLNMP NLNEDQRNAFIQSLKDDPSQ SANVLAEAQKLNDVQAPKA

Data sets:
Data typeCount
13C chemical shifts181
15N chemical shifts69
1H chemical shifts399

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1A219 monomer1

Entities:

Entity 1, A219 monomer 59 residues - Formula weight is not available

1   METTYRTYRLEUVALVALASNLYSGLNGLN
2   ASNALAPHETYRGLUVALLEUASNMETPRO
3   ASNLEUASNGLUASPGLNARGASNALAPHE
4   ILEGLNSERLEULYSASPASPPROSERGLN
5   SERALAASNVALLEUALAGLUALAGLNLYS
6   LEUASNASPVALGLNALAPROLYSALA

Samples:

A219_1: A219, [U-13C; U-15N], 0.2 – 0.4 mM; ammonium bicarbonate 2 mM; postassium phosphate buffer 100 mM; D2O 10%

A219_2: A2190.2 – 0.4 mM; ammonium bicarbonate 2 mM; postassium phosphate buffer 100 mM; D2O 10%

conditions_1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D CBCACONHnot availablenot availablenot available
3D HBHA(CBCACO)NHnot availablenot availablenot available
3D(H)C(CO)NH-TOCSYnot availablenot availablenot available
3DH(CCO)NH-TOCSYnot availablenot availablenot available
2DTOCSYnot availablenot availablenot available
2D-CBHDnot availablenot availablenot available
2DCBHEnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

xwinnmr v2.5, Bruker -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts