BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6686

Title: 1H NMR assignments of the Ce2-TR1C complex at 298 K   PubMed: 9305950

Deposition date: 2005-06-13 Original release date: 2013-02-15

Authors: Hutchinson, John

Citation: Bentrop, Detlef; Bertini, Ivano; Cremonini, Mauro; Forsen, Sture; Luchinat, Claudio; Malmendal, Anders. "Solution Structure of the Paramagnetic Complex of the N-Terminal Domain of Calmodulin with Two Ce3+ Ions by 1H NMR."  Biochemistry 36, 11605-11618 (1997).

Assembly members:
calmodulin n-terminal domain, polymer, 76 residues, Formula weight is not available
Ce2-TR1C, non-polymer, Formula weight is not available

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
calmodulin n-terminal domain: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARK

Data sets:
Data typeCount
1H chemical shifts422

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin n-terminal domain1
2Ce2-TR1C 12
3Ce2-TR1C 22

Entities:

Entity 1, calmodulin n-terminal domain 76 residues - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYTHRILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   THRMETMETALAARGLYS

Entity 2, Ce2-TR1C 1 - Formula weight is not available

1   Ce2-TR1C

Samples:

sample_1: calmodulin n-terminal domain mM

conditions_1: temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

UXNMR, Bruker - NMR data processing

xwinnmr, Bruker - NMR data processing

XEASY, Prof. Kurt Wuthrich - 2D spectra analysis

DYANA, Peter Guntert - structure calculation

CALIBRA, Peter Guntert -

FANTASIA, L. Banci -

NMR spectrometers:

  • Bruker DRX 500 500 MHz
  • Bruker AC-P 300 300 MHz

Related Database Links:

BMRB 18082 18302 4174 6861 6862
PDB
EMBL CAA29381 CAB65357 CCD82979
GB AAH53790 ABW89042 ABW89043 ABW89044 ABW89045
REF XP_001869424 XP_001995129 XP_002772218 XP_005112967 XP_005391108