BMRB Entry 6724
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6724
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Title: Nuclear Magnetic Resonance Structure-Based Epitope Mapping and Modulation of Dust Mite Group 13 Allergen as a Hypoallergen
Deposition date: 2005-07-03 Original release date: 2006-04-05
Authors: Chan, Siew Leong; Mok, Yu-Keung
Citation: Chan, Siew Leong; Ong, Seow Theng; Ong, Su Yin; Chew, Fook Tim; Mok, Yu Keung. "Nuclear Magnetic Resonance Structure-Based Epitope Mapping and Modulation of Dust Mite Group 13 Allergen as a Hypoallergen" J. Immunol. 176, 4852-4860 (2006).
Assembly members:
Der f 13, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: House Dust Mites Taxonomy ID: 6954 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Dermatophagoides farinae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Der f 13: MASIEGKYKLEKSEKFDEFL
DKLGVGFMVKTAAKTLKPTF
EVAIENDQYIFRSLSTFKNT
EAKFKLGEEFEEDRADGKRV
KTVIQKEGDNKFVQTQFGDK
EVKIIREFNGDEVVVTASCD
GVTSVRTYKRI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 397 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 741 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fatty_acid binding protein | 1 |
Entities:
Entity 1, Fatty_acid binding protein 131 residues - Formula weight is not available
| 1 | MET | ALA | SER | ILE | GLU | GLY | LYS | TYR | LYS | LEU | ||||
| 2 | GLU | LYS | SER | GLU | LYS | PHE | ASP | GLU | PHE | LEU | ||||
| 3 | ASP | LYS | LEU | GLY | VAL | GLY | PHE | MET | VAL | LYS | ||||
| 4 | THR | ALA | ALA | LYS | THR | LEU | LYS | PRO | THR | PHE | ||||
| 5 | GLU | VAL | ALA | ILE | GLU | ASN | ASP | GLN | TYR | ILE | ||||
| 6 | PHE | ARG | SER | LEU | SER | THR | PHE | LYS | ASN | THR | ||||
| 7 | GLU | ALA | LYS | PHE | LYS | LEU | GLY | GLU | GLU | PHE | ||||
| 8 | GLU | GLU | ASP | ARG | ALA | ASP | GLY | LYS | ARG | VAL | ||||
| 9 | LYS | THR | VAL | ILE | GLN | LYS | GLU | GLY | ASP | ASN | ||||
| 10 | LYS | PHE | VAL | GLN | THR | GLN | PHE | GLY | ASP | LYS | ||||
| 11 | GLU | VAL | LYS | ILE | ILE | ARG | GLU | PHE | ASN | GLY | ||||
| 12 | ASP | GLU | VAL | VAL | VAL | THR | ALA | SER | CYS | ASP | ||||
| 13 | GLY | VAL | THR | SER | VAL | ARG | THR | TYR | LYS | ARG | ||||
| 14 | ILE |
Samples:
sample_1: Der f 13, [U-15N], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%
sample_2: Der f 13, [U-13C], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%
sample_3: Der f 13, [U-13C; U-15N], 1 ± 0 mM; acetate buffer 50 mM; D2O 10%
conditions_1: pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HCCH-TOCSY | sample_2 | not available | conditions_1 |
| HCCCONH | sample_3 | not available | conditions_1 |
| CBCACONH | sample_3 | not available | conditions_1 |
| HNCACB | sample_3 | not available | conditions_1 |
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts