BMRB Entry 6748
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6748
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Title: NMR assignments of the Ki67FHA/hNIFK(226-269)3P complex
Deposition date: 2005-07-25 Original release date: 2007-01-29
Authors: Byeon, In-Ja; Gronenborn, Angela; Li, Hongyuan; Tsai, Ming-Daw
Citation: Byeon, In-Ja; Li, Hongyuan; Song, Haiyan; Gronenborn, Angela; Tsai, Ming-Daw. "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67." Nat. Struct. Mol. Biol. 12, 987-993 (2005).
Assembly members:
Ki67FHA, polymer, 120 residues, Formula weight is not available
hNIFK, polymer, 120 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ki67FHA: MWPTRRLVTIKRSGVDGPHF
PLSLSTCLFGRGIECDIRIQ
LPVVSKQHCKIEIHEQEAIL
HNFSSTNPTQVNGSVIDEPV
RLKHGDVITIIDRSFRYENE
SLQNGRKSTEFPRKIREQEP
hNIFK: KTVDSQGPTPVCTPTFLERR
KSQVAELNDDDKDDEIVFKQ
PISC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 520 |
| 15N chemical shifts | 168 |
| 1H chemical shifts | 1055 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ki67FHA | 1 |
| 2 | hNIFK | 2 |
Entities:
Entity 1, Ki67FHA 120 residues - Formula weight is not available
| 1 | MET | TRP | PRO | THR | ARG | ARG | LEU | VAL | THR | ILE | |
| 2 | LYS | ARG | SER | GLY | VAL | ASP | GLY | PRO | HIS | PHE | |
| 3 | PRO | LEU | SER | LEU | SER | THR | CYS | LEU | PHE | GLY | |
| 4 | ARG | GLY | ILE | GLU | CYS | ASP | ILE | ARG | ILE | GLN | |
| 5 | LEU | PRO | VAL | VAL | SER | LYS | GLN | HIS | CYS | LYS | |
| 6 | ILE | GLU | ILE | HIS | GLU | GLN | GLU | ALA | ILE | LEU | |
| 7 | HIS | ASN | PHE | SER | SER | THR | ASN | PRO | THR | GLN | |
| 8 | VAL | ASN | GLY | SER | VAL | ILE | ASP | GLU | PRO | VAL | |
| 9 | ARG | LEU | LYS | HIS | GLY | ASP | VAL | ILE | THR | ILE | |
| 10 | ILE | ASP | ARG | SER | PHE | ARG | TYR | GLU | ASN | GLU | |
| 11 | SER | LEU | GLN | ASN | GLY | ARG | LYS | SER | THR | GLU | |
| 12 | PHE | PRO | ARG | LYS | ILE | ARG | GLU | GLN | GLU | PRO |
Entity 2, hNIFK 120 residues - Formula weight is not available
| 1 | LYS | THR | VAL | ASP | SER | GLN | GLY | PRO | THR | PRO | ||||
| 2 | VAL | CYS | THR | PRO | THR | PHE | LEU | GLU | ARG | ARG | ||||
| 3 | LYS | SER | GLN | VAL | ALA | GLU | LEU | ASN | ASP | ASP | ||||
| 4 | ASP | LYS | ASP | ASP | GLU | ILE | VAL | PHE | LYS | GLN | ||||
| 5 | PRO | ILE | SER | CYS |
Samples:
sample_1: Ki67FHA, [U-13C; U-15N], 1 mM; hNIFK 1 mM
sample_2: Ki67FHA 1 mM; hNIFK, [U-13C; U-15N], 1 mM
conditions_1: pH: 7.4; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
Related Database Links:
| DBJ | BAB41210 BAF84592 BAI47080 |
| GB | AAH12457 AAH22990 AAH24238 AAY14830 ABM82604 |
| REF | NP_115766 XP_003818352 XP_004031768 XP_009242042 XP_515769 |
| SP | Q9BYG3 |
| AlphaFold | Q9BYG3 |
Download HSQC peak lists in one of the following formats:
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