BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6805

Title: Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex   PubMed: 16456703

Deposition date: 2005-09-01 Original release date: 2006-03-10

Authors: Dogan, Jakob; Lendel, Christofer; Hard, Torleif

Citation: Dogan, Jakob; Lendel, Christofer; Hard, Torleif. "NMR Assignments of the Free and Bound-state Protein Components of an Anti-idiotypic Affibody Complex"  J. Biomol. NMR 36, 13-13 (2006).

Assembly members:
anti-Ztaq, polymer, 58 residues, 6419.3 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
anti-Ztaq: VDNKFNKERVIAIGEIMRLP NLNSLQVVAFINSLRDDPSQ SANLLAEAKKLNDAQAPK

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts66
1H chemical shifts434

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1anti-Ztaq1

Entities:

Entity 1, anti-Ztaq 58 residues - 6419.3 Da.

1   VALASPASNLYSPHEASNLYSGLUARGVAL
2   ILEALAILEGLYGLUILEMETARGLEUPRO
3   ASNLEUASNSERLEUGLNVALVALALAPHE
4   ILEASNSERLEUARGASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Samples:

sample_1: anti-Ztaq, [U-13C; U-15N], 1.5 mM; Potassium Phosphate 20 mM; Sodium Chloride 50 mM; Sodium Azide 0.01%; D2O 10 % v/v

conditions_1: pH*: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
(H)CC(CO)NHnot availablenot availablenot available
15N-NOESY HSQCnot availablenot availablenot available
15N-TOCSY HSQCnot availablenot availablenot available
13C-NOESY HSQCnot availablenot availablenot available

Software:

ANSIG for WINDOWS - assignment, Data analysis

XWIN-NMR - processing

NMRPipe - processing

NMR spectrometers:

  • BRUKER AVANCE 600 MHz
  • BRUKER AVANCE 500 MHz
  • VARIAN INOVA 800 MHz

Related Database Links:

BMRB 6806
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts