BMRB Entry 6833

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for holo-MazF(E24A) with MazEp(54-77)
Published: 2007-01-29

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6833

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for holo-MazF(E24A) with MazEp(54-77) PubMed: 16413577

Deposition date: 2005-09-21 Original release date: 2007-01-29

Authors: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko

Citation: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko. "Characterization of Dual Substrate Binding Sites in the Homodimeric Structure of Escherichia coli mRNA Interferase MazF" J. Mol. Biol. 357, 139-150 (2006).

Assembly members:
E. COLI TOXIN mRNA interferase, polymer, 114 residues, 12321 Da.
MazEp(54-77), polymer, 24 residues, 2890 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: Protista Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Vector: pET28a

Entity Sequences (FASTA):
E. COLI TOXIN mRNA interferase: GSHMVSRYVPDMGDLIWVDF DPTKGSAQAGHRPAVVLSPF MYNNKTGMCLCVPCTTQSKG YPFEVVLSGQERDGVALADQ VKSIAWRARGATKKGTVAPE ELQLIKAKINVLIG
MazEp(54-77): TLAELVNDITPENLHENIDW GEPK

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	212
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MazF(E24A)	1
2	MazF(E24A)	1
3	MazEp(54-77)	2
4	MazEp(54-77)	2

Entities:

Entity 1, MazF(E24A) 114 residues - 12321 Da.

1

GLY

SER

HIS

MET

VAL

SER

ARG

TYR

VAL

PRO

2

ASP

MET

GLY

ASP

LEU

ILE

TRP

VAL

ASP

PHE

3

ASP

PRO

THR

LYS

GLY

SER

ALA

GLN

ALA

GLY

4

HIS

ARG

PRO

ALA

VAL

LEU

SER

PRO

PHE

5

MET

TYR

ASN

LYS

THR

GLY

MET

CYS

LEU

6

CYS

VAL

PRO

CYS

THR

GLN

SER

LYS

GLY

7

TYR

PRO

PHE

GLU

VAL

LEU

SER

GLY

GLN

8

GLU

ARG

ASP

GLY

VAL

ALA

LEU

ALA

ASP

GLN

9

VAL

LYS

SER

ILE

ALA

TRP

ARG

ALA

ARG

GLY

10

ALA

THR

LYS

GLY

THR

VAL

ALA

PRO

GLU

11

GLU

LEU

GLN

LEU

ILE

LYS

ALA

LYS

ILE

ASN

12

VAL

LEU

ILE

GLY

Entity 2, MazEp(54-77) 24 residues - 2890 Da.

1

THR

LEU

ALA

GLU

LEU

VAL

ASN

ASP

ILE

THR

2

PRO

GLU

ASN

LEU

HIS

GLU

ASN

ILE

ASP

TRP

3

GLY

GLU

PRO

LYS

Samples:

sample_1: MazF(E24A) mutant, [U-95% 13C; U-95% 15N], 0.8 ± 0.1 mM; MazEp(54-77) 1.5 ± 0.1 mM; NaPi 20 mM; NaCl 100 mM; DTT 5 mM; TCEP 2 mM; NaN3 1 mM; AEBSF 1 mM; D2O 10%; H2O 90%

conditions_1: pH: 7.00; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
CBCACONNH	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1

Software:

XEASY v1.3 -

NMR spectrometers:

Varian Unity-Inova 600 MHz

PDB	1MVF 1UB4
DBJ	BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL	CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB	AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF	NP_289337 NP_311670 NP_417263 WP_000581935 WP_000581937
SP	P0AE72 P0AE73
AlphaFold	P0AE72 P0AE73

Biological Magnetic Resonance Data Bank