BMRB Entry 6833

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6833

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for holo-MazF(E24A) with MazEp(54-77)
Deposition date:
2005-09-21
Original release date:
2007-01-29
Authors:
Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko
Citation:

Citation: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko. "Characterization of Dual Substrate Binding Sites in the Homodimeric Structure of Escherichia coli mRNA Interferase MazF"  J. Mol. Biol. 357, 139-150 (2006).
PubMed: 16413577

Assembly members:

Assembly members:
E. COLI TOXIN mRNA interferase, polymer, 114 residues, 12321 Da.
MazEp(54-77), polymer, 24 residues, 2890 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: Protista   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E. COLI TOXIN mRNA interferase: GSHMVSRYVPDMGDLIWVDF DPTKGSAQAGHRPAVVLSPF MYNNKTGMCLCVPCTTQSKG YPFEVVLSGQERDGVALADQ VKSIAWRARGATKKGTVAPE ELQLIKAKINVLIG
MazEp(54-77): TLAELVNDITPENLHENIDW GEPK

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts101
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MazF(E24A)1
2MazF(E24A)1
3MazEp(54-77)2
4MazEp(54-77)2

Entities:

Entity 1, MazF(E24A) 114 residues - 12321 Da.

1   GLYSERHISMETVALSERARGTYRVALPRO
2   ASPMETGLYASPLEUILETRPVALASPPHE
3   ASPPROTHRLYSGLYSERALAGLNALAGLY
4   HISARGPROALAVALVALLEUSERPROPHE
5   METTYRASNASNLYSTHRGLYMETCYSLEU
6   CYSVALPROCYSTHRTHRGLNSERLYSGLY
7   TYRPROPHEGLUVALVALLEUSERGLYGLN
8   GLUARGASPGLYVALALALEUALAASPGLN
9   VALLYSSERILEALATRPARGALAARGGLY
10   ALATHRLYSLYSGLYTHRVALALAPROGLU
11   GLULEUGLNLEUILELYSALALYSILEASN
12   VALLEUILEGLY

Entity 2, MazEp(54-77) 24 residues - 2890 Da.

1   THRLEUALAGLULEUVALASNASPILETHR
2   PROGLUASNLEUHISGLUASNILEASPTRP
3   GLYGLUPROLYS

Related Database Links:

PDB 1MVF 1UB4
DBJ BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF NP_289337 NP_311670 NP_417263 WP_000581935 WP_000581937
SP P0AE72 P0AE73
AlphaFold P0AE72 P0AE73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks