BMRB Entry 6840

Name: 15N 13C and 1H chemical shift assignments for the WIF domain of human WIF-1
Published: 2006-04-24

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6840

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

15N 13C and 1H chemical shift assignments for the WIF domain of human WIF-1

Deposition date:

2005-09-29

Original release date:

2006-04-24

Authors:

Liepinsh, Edvards; Otting, Gottfried

Citation:

Citation: Liepinsh, Edvards; Banyai, Laszlo; Patthy, Laszlo; Otting, Gottfried. "NMR Structure of the WIF Domain of the Human Wnt-Inhibitory Factor-1." J. Mol. Biol. 357, 942-950 (2006).
PubMed: 16476441

Assembly members:

Assembly members:
WIF domain, polymer, 157 residues, 17495 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WIF domain: GSHMLDQQEESLYLWIDAHQ ARVLIGFEEDILIVSEGKMA PFTHDFRKAQQRMPAIPVNI HSMNFTWQAAGQAEYFYEFL SLRSLDKGIMADPTVNVPLL GTVPHKASVVQVGFPCLGKQ DGVAAFEVDVIVMNSEGNTI LQTPQNAIFFKTCLQAE

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	430
15N chemical shifts	167
1H chemical shifts	1105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WIF domain	1

Entities:

Entity 1, WIF domain 157 residues - 17495 Da.

1

GLY

SER

HIS

MET

LEU

ASP

GLN

GLU

2

SER

LEU

TYR

LEU

TRP

ILE

ASP

ALA

HIS

GLN

3

ALA

ARG

VAL

LEU

ILE

GLY

PHE

GLU

ASP

4

ILE

LEU

ILE

VAL

SER

GLU

GLY

LYS

MET

ALA

5

PRO

PHE

THR

HIS

ASP

PHE

ARG

LYS

ALA

GLN

6

GLN

ARG

MET

PRO

ALA

ILE

PRO

VAL

ASN

ILE

7

HIS

SER

MET

ASN

PHE

THR

TRP

GLN

ALA

8

GLY

GLN

ALA

GLU

TYR

PHE

TYR

GLU

PHE

LEU

9

SER

LEU

ARG

SER

LEU

ASP

LYS

GLY

ILE

MET

10

ALA

ASP

PRO

THR

VAL

ASN

VAL

PRO

LEU

11

GLY

THR

VAL

PRO

HIS

LYS

ALA

SER

VAL

12

GLN

VAL

GLY

PHE

PRO

CYS

LEU

GLY

LYS

GLN

13

ASP

GLY

VAL

ALA

PHE

GLU

VAL

ASP

VAL

14

ILE

VAL

MET

ASN

SER

GLU

GLY

ASN

THR

ILE

15

LEU

GLN

THR

PRO

GLN

ASN

ALA

ILE

PHE

16

LYS

THR

CYS

LEU

GLN

ALA

GLU

Samples:

sample_1: WIF domain, [U-15N], 0.4 ± 0.1 mM

sample_2: WIF domain, [U-15N; U-13C], 0.7 ± 0.1 mM

conditions_1: pH: 6.0; pressure: 1 bar; temperature: 301 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
2D NOESY	not available	not available	conditions_1
HNCA	sample_2	not available	conditions_1
CBCA(CO)NH	sample_2	not available	conditions_1
HNCO	sample_2	not available	conditions_1
3D NOESY-15N-HSQC	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker DMX 600 MHz
Varian Unity 800 MHz

PDB	2D3J 2YGQ
DBJ	BAE25067 BAE34471 BAG63265
GB	AAD25402 AAD25403 AAH13268 AAH18037 AAQ88710
REF	NP_001247600 NP_009122 NP_036045 XP_001163469 XP_001490647
SP	Q9WUA1 Q9Y5W5
AlphaFold	Q9WUA1 Q9Y5W5