BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6854

Title: 1H, 13C, and 15N Chemical Shift Assignments for the first SH3 domain of Nck2   PubMed: 16604428

Deposition date: 2005-10-07 Original release date: 2006-05-02

Authors: Park, Sunghyouk; Takeuchi, Koh; Wagner, Gerhard

Citation: Park, Sunghyouk; Takeuchi, Koh; Wagner, Gerhard. "Solution structure of the first Src Homology 3 domain of Nck2"  J. Biomol. NMR 34, 203-208 (2006).

Assembly members:
Nck SH3 domain (1st), polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Nck SH3 domain (1st): MTEEVIVIAKWDYTAQQDQE LDIKKNERLWLLDDSKTWWR VRNAANRTGYVPSNYVERK

Data sets:
Data typeCount
13C chemical shifts182
15N chemical shifts67
1H chemical shifts408

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1the first SH3 domain of Nck1

Entities:

Entity 1, the first SH3 domain of Nck 59 residues - Formula weight is not available

1   METTHRGLUGLUVALILEVALILEALALYS
2   TRPASPTYRTHRALAGLNGLNASPGLNGLU
3   LEUASPILELYSLYSASNGLUARGLEUTRP
4   LEULEUASPASPSERLYSTHRTRPTRPARG
5   VALARGASNALAALAASNARGTHRGLYTYR
6   VALPROSERASNTYRVALGLUARGLYS

Samples:

sample_1: Nck SH3 domain (1st), [U-13C; U-15N], 0.6 mM; Sodium Phosphate 50 mM; EDTA 50 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
13C edited NOESYsample_1not availableconditions_1
15N edited NOESYsample_1not availableconditions_1
HCCH TOCSYsample_1not availableconditions_1
D2O TOCSYsample_1not availableconditions_1
D2O NOESYsample_1not availableconditions_1
HCCONHsample_1not availableconditions_1
CCONHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAY14937 EPQ18600 EPY89463
REF NP_001004722 XP_001516770 XP_005037783 XP_005276048 XP_005575245

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts