BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6867

Title: Solution structure of micelle-bound fusion domain of HIV-1 gp41   PubMed: 16331977

Deposition date: 2005-10-20 Original release date: 2005-12-28

Authors: Jaroniec, C.; Kaufman, J.; Stahl, S.; Viard, M.; Blumenthal, R.; Wingfield, P.; Bax, A.

Citation: Jaroniec, C.; Kaufman, J.; Stahl, S.; Viard, M.; Blumenthal, R.; Wingfield, P.; Bax, A.. "Structure and Dynamics of Micelle-Associated Human Immunodeficiency Virus gp41 Fusion Domain"  Biochemistry 44, 16167-16180 (2005).

Assembly members:
Envelope polyprotein GP160, polymer, 39 residues, Formula weight is not available

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Envelope polyprotein GP160: PAVGIGALFLGFLGAAGSTM GAASMTLTVQADYKDDDDK

Data sets:
Data typeCount
13C chemical shifts110
15N chemical shifts38
1H chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Envelope polyprotein GP1601

Entities:

Entity 1, Envelope polyprotein GP160 39 residues - Formula weight is not available

1   PROALAVALGLYILEGLYALALEUPHELEU
2   GLYPHELEUGLYALAALAGLYSERTHRMET
3   GLYALAALASERMETTHRLEUTHRVALGLN
4   ALAASPTYRLYSASPASPASPASPLYS

Samples:

sample_1: Envelope polyprotein GP160, [U-2H; U-13C; U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%

sample_2: Envelope polyprotein GP160, [U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%

sample_3: Envelope polyprotein GP160, [U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%; polyacryalmide gel

sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HN(CA)CBnot availablenot availablenot available
2D HNCGnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

xwinnmr v2.6 & 3.5, Bruker - collection

NMRPipe v2.3, Delaglio - data analysis, processing

SPARKY v3.11, Goddard - data analysis

X-PLOR NIH v2.9.4, Schwieters - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAD66665 BAE96228 BAE96229 BAE96230 BAE96231
EMBL CAA25903 CAA43618 CAA43620 CAA77628
GB AAA44205 AAA44661 AAA44679 AAA44850 AAA44992
PRF 1102247A 1103299B 1923361A 2207165U 2207165V
REF NP_057856 NP_579895
SP P03375 P04578 P04582 P04624 P19551
AlphaFold P04624 P03375 P04578 P04582 P19551

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts