BMRB Entry 6904
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6904
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C and 15N resonance assignment of M-crystallin: a novel Ca2+ binding protein of the bg-crystallin superfamily from Methanosarcina acetivorans PubMed: 16607466
Deposition date: 2005-11-17 Original release date: 2008-09-02
Authors: Barnwal, Ravi; Jobby, M.; Sharma, Yogendra; Chary, Kandala
Citation: Barnwal, Ravi; Jobby, M.; Sharma, Yogendra; Chary, Kandala. "NMR Assignment of M-crystallin: A Novel Ca(2+) Binding Protein of the betagamma-crystallin Superfamily from Methanosarcina acetivorans." J. Biomol. NMR 36, 32-32 (2006).
Assembly members:
M-Crystallin, polymer, 85 residues, 9376.2 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Methanosarcina acetivorans Taxonomy ID: 2214 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina acetivorans
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
M-Crystallin: MNAAEVIVYEHVNFGGKSFD
ATSDQPGAGDNLNDKISSIK
VKSGTWRFYEYINYGGRYWD
LGPGEYSSVESAGIPDNSIS
SFRQI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 311 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 509 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | M-Crystallin | 1 |
| 2 | CALCIUM (II) ION | 2 |
Entities:
Entity 1, M-Crystallin 85 residues - 9376.2 Da.
| 1 | MET | ASN | ALA | ALA | GLU | VAL | ILE | VAL | TYR | GLU | ||||
| 2 | HIS | VAL | ASN | PHE | GLY | GLY | LYS | SER | PHE | ASP | ||||
| 3 | ALA | THR | SER | ASP | GLN | PRO | GLY | ALA | GLY | ASP | ||||
| 4 | ASN | LEU | ASN | ASP | LYS | ILE | SER | SER | ILE | LYS | ||||
| 5 | VAL | LYS | SER | GLY | THR | TRP | ARG | PHE | TYR | GLU | ||||
| 6 | TYR | ILE | ASN | TYR | GLY | GLY | ARG | TYR | TRP | ASP | ||||
| 7 | LEU | GLY | PRO | GLY | GLU | TYR | SER | SER | VAL | GLU | ||||
| 8 | SER | ALA | GLY | ILE | PRO | ASP | ASN | SER | ILE | SER | ||||
| 9 | SER | PHE | ARG | GLN | ILE |
Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
Sample_1: M-Crystallin, [U-99% 15N], 1.1 mM; CALCIUM (II) ION 6.6 mM
Sample_2: M-Crystallin, [U-99% 13C; U-99% 15N], 0.9 mN; CALCIUM (II) ION 5.4 mM
Cond_1: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | Cond_1 |
| HN(CA)CO | not available | not available | Cond_1 |
| CBCANH | not available | not available | Cond_1 |
| CBCA(CO)NH | not available | not available | Cond_1 |
| 1H-15N TOCSY-HSQC | not available | not available | Cond_1 |
| 1H-15N NOESY-HSQC | not available | not available | Cond_1 |
Software:
FELIX v97 software -
TATAPRO v97 software -
NMR spectrometers:
- Bruker Biospin 800 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts