BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6909

Title: 1H and 15N Chemical Shift Assignments for the Alpha-domain of Human Metallothionein-3   PubMed: 16413543

Deposition date: 2005-11-25 Original release date: 2007-02-06

Authors: Wang, Hui; Zhang, Qi; Cai, Bin; Li, Hongyan; Sze, Kong-Hung; Huang, Zhong-Xian; Wu, Hou-Ming; Sun, Hongzhe

Citation: Wang, Hui; Zhang, Qi; Cai, Bin; Li, Hongyan; Sze, Kong-Hung; Huang, Zhong-Xian; Wu, Hou-Ming; Sun, Hongzhe. "Solution structure and dynamics of human metallothionein-3 (MT-3)"  FEBS Lett. 580, 795-800 (2006).

Assembly members:
human MT-3, polymer, 68 residues, 6923 Da.
CD, non-polymer, 112.411 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Saccharomyces Homo sapiens

Experimental source:   Production method: recombinant technology   Vector: pGEX4T2

Entity Sequences (FASTA):
human MT-3: MDPETCPCPSGGSCTCADSC KCEGCKCTSCKKSCCSCCPA ECEKCAKDCVCKGGEAAEAE AEKCSCCQ

Data sets:
Data typeCount
15N chemical shifts33
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Metallothionein-31
2Cadmium (II) ion, I2
3Cadmium (II) ion, II2
4Cadmium (II) ion, III2
5Cadmium (II) ion, IV2
6Cadmium (II) ion, V2
7Cadmium (II) ion, VI2
8Cadmium (II) ion, VII2

Entities:

Entity 1, Human Metallothionein-3 68 residues - 6923 Da.

1   METASPPROGLUTHRCYSPROCYSPROSER
2   GLYGLYSERCYSTHRCYSALAASPSERCYS
3   LYSCYSGLUGLYCYSLYSCYSTHRSERCYS
4   LYSLYSSERCYSCYSSERCYSCYSPROALA
5   GLUCYSGLULYSCYSALALYSASPCYSVAL
6   CYSLYSGLYGLYGLUALAALAGLUALAGLU
7   ALAGLULYSCYSSERCYSCYSGLN

Entity 2, Cadmium (II) ion, I - Cd - 112.411 Da.

1   CD

Samples:

sample_1: human MT-3, [U-15N], 2 mM; Phosphate Na 15 mM

conditions_1: pH: 7.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-15N_HSQCsample_1not availableconditions_1
2D_15N-decoupled_TOCSYsample_1not availableconditions_1
2D_15N-decoupled_NOESYsample_1not availableconditions_1
3D_1H-1H-15N-TOCSYsample_1not availableconditions_1
3D_1H-1H-15N-NOESYsample_1not availableconditions_1

Software:

SPARKY v3.0 - peak assignments

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts