BMRB Entry 6912
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6912
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Pinheiro, Anderson; Amorim, Gisele; Netto, Luis Eduardo; Valente, Ana Paula; Almeida, Fabio. "1H, 13C and 15N Resonance Assignments for the Reduced Forms of Thioredoxin 1
and 2 from S. cerevisiae" J. Biomol. NMR 36, 35-35 (2006).
PubMed: 16609834
Assembly members:
Trx1, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Trx1: MVTQFKTASEFDSAIAQDKL
VVVDFYATWCGPCKMIAPMI
EKFSEQYPQADFYKLDVDEL
GDVAQKNEVSAMPTLLLFKN
GKEVAKVVGANPAAIKQAIA
ANA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 696 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yeast thioredoxin 1 | 1 |
Entities:
Entity 1, yeast thioredoxin 1 103 residues - Formula weight is not available
| 1 | MET | VAL | THR | GLN | PHE | LYS | THR | ALA | SER | GLU | ||||
| 2 | PHE | ASP | SER | ALA | ILE | ALA | GLN | ASP | LYS | LEU | ||||
| 3 | VAL | VAL | VAL | ASP | PHE | TYR | ALA | THR | TRP | CYS | ||||
| 4 | GLY | PRO | CYS | LYS | MET | ILE | ALA | PRO | MET | ILE | ||||
| 5 | GLU | LYS | PHE | SER | GLU | GLN | TYR | PRO | GLN | ALA | ||||
| 6 | ASP | PHE | TYR | LYS | LEU | ASP | VAL | ASP | GLU | LEU | ||||
| 7 | GLY | ASP | VAL | ALA | GLN | LYS | ASN | GLU | VAL | SER | ||||
| 8 | ALA | MET | PRO | THR | LEU | LEU | LEU | PHE | LYS | ASN | ||||
| 9 | GLY | LYS | GLU | VAL | ALA | LYS | VAL | VAL | GLY | ALA | ||||
| 10 | ASN | PRO | ALA | ALA | ILE | LYS | GLN | ALA | ILE | ALA | ||||
| 11 | ALA | ASN | ALA |
Samples:
sample_1: thioredoxin 2, [U-13C; U-15N], 1 mM; 2-mercaptoethanol 3 mM; H2O 90%; D2O 10%
sample_2: thioredoxin 2, [U-15N], 1.2 mM; DTT 3 mM; H2O 90%; D2O 10%
sample_3: thioredoxin 2 1.2 mM; DTT 3 mM; D2O 99%
conditions_1: pH: 7.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | conditions_1 |
| HN(CO)CA | not available | not available | conditions_1 |
| HNCACB | not available | not available | conditions_1 |
| CBCA(CO)NH | not available | not available | conditions_1 |
| HNCO | not available | not available | conditions_1 |
| H(CCO)NH-TOCSY | not available | not available | conditions_1 |
| (H)CC(CO)NH-TOCSY | not available | not available | conditions_1 |
| HCCH-TOCSY | not available | not available | conditions_1 |
| 1H-1H_TOCSY_2D | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA24928 |
| EMBL | CAA97572 CAY81281 |
| GB | AAA35171 AAA35177 AAS56529 AHY78447 EDN59589 |
| REF | NP_013144 |
| SP | P22217 |
| TPG | DAA09361 |
| AlphaFold | P22217 |
Download HSQC peak lists in one of the following formats:
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