BMRB Entry 6932
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6932
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Title: Chemical shift assignments of phosphoryl carrier domain of pyruvate phosphate dikinase PubMed: 16460017
Deposition date: 2006-01-06 Original release date: 2006-05-02
Authors: Ames, James
Citation: Lin, Ying; Lusin, Jacqueline; Ye, Dongmei; Dunaway-Mariano, Debra; Ames, James. "Examination of the structure, stability, and catalytic potential in the engineered phosphoryl carrier domain of pyruvate phosphate dikinase." Biochemistry 45, 1702-1711 (2006).
Assembly members:
cent-I, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: C. symbiosum Taxonomy ID: 1512 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium symbiosum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
cent-I: AALKAGEVIGSALPASPGAA
AGKVYFTADEAKAAHEKGER
VILVRLETSPEDIEGMHAAE
GILTVRGGMTSHAAVVARGM
GTCCVSGCGEIKINEEAKTF
ELGGHTFAEGDYISLDGSTG
KIYKGDIE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 491 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 724 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cent-I | 1 |
Entities:
Entity 1, cent-I 128 residues - Formula weight is not available
sequence of the central domain (cent-I) begins at Ala384 and ends at Glu511
| 1 | ALA | ALA | LEU | LYS | ALA | GLY | GLU | VAL | ILE | GLY | ||||
| 2 | SER | ALA | LEU | PRO | ALA | SER | PRO | GLY | ALA | ALA | ||||
| 3 | ALA | GLY | LYS | VAL | TYR | PHE | THR | ALA | ASP | GLU | ||||
| 4 | ALA | LYS | ALA | ALA | HIS | GLU | LYS | GLY | GLU | ARG | ||||
| 5 | VAL | ILE | LEU | VAL | ARG | LEU | GLU | THR | SER | PRO | ||||
| 6 | GLU | ASP | ILE | GLU | GLY | MET | HIS | ALA | ALA | GLU | ||||
| 7 | GLY | ILE | LEU | THR | VAL | ARG | GLY | GLY | MET | THR | ||||
| 8 | SER | HIS | ALA | ALA | VAL | VAL | ALA | ARG | GLY | MET | ||||
| 9 | GLY | THR | CYS | CYS | VAL | SER | GLY | CYS | GLY | GLU | ||||
| 10 | ILE | LYS | ILE | ASN | GLU | GLU | ALA | LYS | THR | PHE | ||||
| 11 | GLU | LEU | GLY | GLY | HIS | THR | PHE | ALA | GLU | GLY | ||||
| 12 | ASP | TYR | ILE | SER | LEU | ASP | GLY | SER | THR | GLY | ||||
| 13 | LYS | ILE | TYR | LYS | GLY | ASP | ILE | GLU |
Samples:
sample_1: cent-I, [U-13C; U-15N], 1.0 mM; KCL 50 mM; potassium phosphate 20 mM
conditions_1: ionic strength: 0.07 M; pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNCOCACB | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HBHACONH | sample_1 | not available | conditions_1 |
| HCC-TOCSY | sample_1 | not available | conditions_1 |
| CCONH-TOCSY | sample_1 | not available | conditions_1 |
| HCCONH-TOCSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance DRX 600 MHz
Related Database Links:
| PDB | |
| GB | AAA22917 EGA95273 EGB19009 EHF07270 ERI77319 |
| REF | WP_003498777 |
| SP | P22983 |
| AlphaFold | P22983 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts